The CYCLIN-DEPENDENT KINASE Module of the Mediator Complex Promotes Flowering and Reproductive Development in Pea.,Plant Physiology

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The CYCLIN-DEPENDENT KINASE Module of the Mediator Complex Promotes Flowering and Reproductive Development in Pea.
Plant Physiology ( IF 6.5 ) Pub Date : 2020-01-21 , DOI: 10.1104/pp.19.01173
A S M Mainul Hasan ₁ , Jacqueline K Vander Schoor ₁ , Valerie Hecht ₁ , James L Weller ₂

Affiliation

Control of flowering time has been a major focus of comparative genetic analyses in plant development. This study reports on a forward genetic approach to define previously uncharacterized components of flowering control pathways in the long-day legume, pea (Pisum sativum). We isolated two complementation groups of late-flowering mutants in pea that define two uncharacterized loci, LATE BLOOMER3 (LATE3) and LATE4, and describe their diverse effects on vegetative and reproductive development. A map-based comparative approach was employed to identify the underlying genes for both loci, revealing that that LATE3 and LATE4 are orthologs of CYCLIN DEPENDENT KINASE8 (CDK8) and CYCLIN C1 (CYCC1), components of the CDK8 kinase module of the Mediator complex, which is a deeply conserved regulator of transcription in eukaryotes. We confirm the genetic and physical interaction of LATE3 and LATE4 and show that they contribute to the transcriptional regulation of key flowering genes, including the induction of the florigen gene FTa1 and repression of the floral repressor LF Our results establish the conserved importance of the CDK8 module in plants and provide evidence for the function of CYCLIN C1 orthologs in the promotion of flowering and the maintenance of normal reproductive development.

中文翻译：

介导复合物的细胞周期蛋白依赖激酶模块促进豌豆开花和生殖发育。

控制开花时间一直是植物发育中比较遗传分析的一个主要焦点。这项研究报告了一种正向遗传方法，用于定义长日照豆科植物豌豆（Pisum sativum）开花控制途径中以前未表征的成分。我们在豌豆中分离了两个互补组的晚花突变体，它们定义了两个未表征的基因座：LATE BLOOMER3 (LATE3) 和 LATE4，并描述了它们对营养和生殖发育的不同影响。采用基于图谱的比较方法来鉴定两个基因座的潜在基因，揭示LATE3和LATE4是CYCLIN DEPENDENT KINASE8 (CDK8)和CYCLIN C1 (CYCC1)的直向同源物，CYCLIN DEPENDENT KINASE8 (CDK8)和CYCLIN C1 (CYCC1)是介导复合物的CDK8激酶模块的组成部分，它是真核生物中高度保守的转录调节因子。我们确认了 LATE3 和 LATE4 的遗传和物理相互作用，并表明它们有助于关键开花基因的转录调控，包括成花基因 FTa1 的诱导和成花抑制基因 LF 的抑制。我们的结果证实了 CDK8 模块的保守重要性并为 CYCLIN C1 直系同源物在促进开花和维持正常生殖发育中的功能提供证据。

更新日期：2020-03-03

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