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Fly-on-a-Chip: Microfluidics for Drosophila melanogaster Studies.
Integrative Biology ( IF 1.5 ) Pub Date : 2019-12-31 , DOI: 10.1093/intbio/zyz037
Alireza Zabihihesari 1 , Arthur J Hilliker 2 , Pouya Rezai 1
Affiliation  

The fruit fly or Drosophila melanogaster has been used as a promising model organism in genetics, developmental and behavioral studies as well as in the fields of neuroscience, pharmacology, and toxicology. Not only all the developmental stages of Drosophila, including embryonic, larval, and adulthood stages, have been used in experimental in vivo biology, but also the organs, tissues, and cells extracted from this model have found applications in in vitro assays. However, the manual manipulation, cellular investigation and behavioral phenotyping techniques utilized in conventional Drosophila-based in vivo and in vitro assays are mostly time-consuming, labor-intensive, and low in throughput. Moreover, stimulation of the organism with external biological, chemical, or physical signals requires precision in signal delivery, while quantification of neural and behavioral phenotypes necessitates optical and physical accessibility to Drosophila. Recently, microfluidic and lab-on-a-chip devices have emerged as powerful tools to overcome these challenges. This review paper demonstrates the role of microfluidic technology in Drosophila studies with a focus on both in vivo and in vitro investigations. The reviewed microfluidic devices are categorized based on their applications to various stages of Drosophila development. We have emphasized technologies that were utilized for tissue- and behavior-based investigations. Furthermore, the challenges and future directions in Drosophila-on-a-chip research, and its integration with other advanced technologies, will be discussed.

中文翻译:

芯片飞行:果蝇研究的微流控技术。

果蝇或果蝇已经在遗传学,发育和行为研究以及神经科学,药理学和毒理学领域用作有希望的模式生物。不仅果蝇的所有发育阶段,包括胚胎,幼虫和成年阶段,都已用于实验体内生物学,而且从该模型中提取的器官,组织和细胞已在体外测定中得到应用。但是,在基于果蝇的常规体内和体外测定中使用的手动操作,细胞研究和行为表型技术大多耗时,费力且通量低。此外,用外部生物,化学或物理信号刺激生物需要精确的信号传递,而对神经和行为表型的量化需要果蝇的光学和物理可达性。最近,微流控和芯片实验室设备已经成为克服这些挑战的强大工具。这篇综述文章证明了微流体技术在果蝇研究中的作用,重点是体内和体外研究。审查的微流控设备根据其在果蝇发育各个阶段的应用进行分类。我们强调了用于组织和行为调查的技术。此外,还将讨论果蝇片上研究及其与其他先进技术的集成所面临的挑战和未来方向。微流体和芯片实验室设备已经成为克服这些挑战的强大工具。这篇综述文章证明了微流体技术在果蝇研究中的作用,重点是体内和体外研究。审查的微流控设备根据其在果蝇发育各个阶段的应用进行分类。我们强调了用于基于组织和行为的研究的技术。此外,还将讨论果蝇片上研究及其与其他先进技术的集成所面临的挑战和未来方向。微流体和芯片实验室设备已经成为克服这些挑战的强大工具。这篇综述文章证明了微流体技术在果蝇研究中的作用,重点是体内和体外研究。审查的微流控设备根据其在果蝇发育各个阶段的应用进行分类。我们强调了用于组织和行为调查的技术。此外,还将讨论果蝇片上研究及其与其他先进技术的集成所面临的挑战和未来方向。本文综述了果蝇研究中的微流体技术的作用,重点是体内和体外研究。审查的微流控设备根据其在果蝇发育各个阶段的应用进行分类。我们强调了用于组织和行为调查的技术。此外,还将讨论果蝇片上研究及其与其他先进技术的集成所面临的挑战和未来方向。这篇综述文章证明了微流体技术在果蝇研究中的作用,重点是体内和体外研究。审查的微流控设备根据其在果蝇发育各个阶段的应用进行分类。我们强调了用于组织和行为调查的技术。此外,还将讨论果蝇片上研究及其与其他先进技术的集成所面临的挑战和未来方向。
更新日期:2020-01-22
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