Ionic liquid decorated AIE luminogen for selective detection of HSA in biofluids and early disease screening.,Talanta

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Ionic liquid decorated AIE luminogen for selective detection of HSA in biofluids and early disease screening.
Talanta ( IF 5.6 ) Pub Date : 2020-01-20 , DOI: 10.1016/j.talanta.2020.120763
Lifang Gao ₁ , Xin Lin ₁ , Xuwei Chen ₁

Affiliation

The precise evaluation of human serum albumin (HSA) contents in biofluids is critical due to its important physiological functions. A fluorescent luminogen TPE-IL is facilely fabricated in present study by decorating the aggregation induced emission (AIE) dye E-1,2-bis(4-hydroxyphenyl)-1,2-diphenylethylene (TPE-OH) with ionic liquid (IL) HOOCMIMBr. The hydrophobic and hydrogen-bond interactions between the amino acid residues of HSA and TPE-IL lead to the spontaneous and energetically favorable docking of TPE-IL molecules in the hydrophobic subdomain of HSA, inducing the significant fluorescence enhancement of this sensor. Highly sensitive and selective detection of HSA is accomplished in the linear range of 0.02-10 μg/mL, with a detection limit of 0.007 μg/mL. The practicability of this sensor is validated by the accurate detection of HSA contents in human serum and urine samples. A glass slide-based visual sensing platform is also constructed to offer simple and fast HSA content evaluation in serum for early disease screening.

中文翻译：

离子液体修饰的AIE发光剂，可选择性检测生物流体中的HSA和早期疾病筛查。

由于其重要的生理功能，因此准确评估生物流体中人血清白蛋白（HSA）的含量至关重要。通过用离子液体（IL）装饰聚集诱导发射（AIE）染料E-1,2-双（4-羟基苯基）-1,2-二苯基乙烯（TPE-OH），可以轻松地制造荧光致发光剂TPE-IL ）HOOCMIMBr。HSA和TPE-IL氨基酸残基之间的疏水和氢键相互作用导致TPE-IL分子在HSA疏水子域中自发且在能量上有利于对接，从而导致该传感器的荧光显着增强。HSA的高灵敏度和选择性检测在0.02-10μg/ mL的线性范围内完成，检测极限为0.007μg/ mL。该传感器的实用性通过准确检测人血清和尿液样品中HSA含量而得到验证。还构建了基于载玻片的视觉传感平台，以提供简单，快速的血清中HSA含量评估，以进行早期疾病筛查。

更新日期：2020-01-21

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