Signal-off/on Electrogenerated Chemiluminescence Deoxyribosensors for Assay of Early Lung Cancer Biomarker (NAP2) Based on Target-caused DNA Charge Transfer,Analytica Chimica Acta

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Signal-off/on Electrogenerated Chemiluminescence Deoxyribosensors for Assay of Early Lung Cancer Biomarker (NAP2) Based on Target-caused DNA Charge Transfer
Analytica Chimica Acta ( IF 5.7 ) Pub Date : 2020-03-01 , DOI: 10.1016/j.aca.2019.12.049
Yu Chen ₁ , Lina Sun ₁ , Xinrui Qiao ₁ , Yuanfu Zhang ₂ , Yan Li ₁ , Fen Ma ₁

Affiliation

Two novel electrochemiluminescence (ECL) deoxyribosensors are designed for assay of early lung cancer biomarker (NAP2) using the DNA three-way junction (DNA-TWJ) inserted NAP2 binding aptamer between two double-helical stems and labeled with ruthenium (II) complex (Ru) (NBAT-Ru) taken as molecular recognition element. The signal-off ECL deoxyribosensor was fabricated by covalently coupling the 5'-NH2-(CH2)6-NBAT-Ru to glassy carbon electrode surface modified with 4-aminobenzoic acid (4-ABA). After combining NAP2 and NBAT-Ru, the changed conformation of NBAT-Ru altered the distance between Ru complex and electrode, resulting in a low ECL signal. The signal-on deoxyribosensor was fabricated by self-assembling the 5'-SH-(CH2)6-NBAT-Ru onto the Au electrode. The introduction of NAP2 triggered the conformational change in the aptamer domain, which induces the interhelical stacking of the two double-helical stems of NBAT-Ru. This stacking constitutes "electrical contact," which promotes transmission of electron-holes through the stems of NBAT-Ru, and produces high ECL intensity. Both deoxyribosensors show high sensitivity and selectivity. The biosensors have been successfully applied to clinical plasma detection. The approaches we describe represent unique principles based on DNA-TWJ inserted target special binding domain as molecular recognition element and different immobilization types for the fabrication of biosensors, which are greatly promising for the detection of protein, metal ions, bacteria, and cells.

中文翻译：

基于目标引起的 DNA 电荷转移的用于检测早期肺癌生物标志物 (NAP2) 的电生化学发光脱氧核糖传感器的信号关闭/开启

设计了两种新型电化学发光 (ECL) 脱氧核糖传感器，使用 DNA 三路连接 (DNA-TWJ) 在两个双螺旋茎之间插入 NAP2 结合适体并用钌 (II) 复合物标记，用于测定早期肺癌生物标志物 (NAP2)。 Ru) (NBAT-Ru) 作为分子识别元素。通过将 5'-NH2-(CH2)6-NBAT-Ru 共价偶联到用 4-氨基苯甲酸 (4-ABA) 修饰的玻碳电极表面来制造信号关闭 ECL 脱氧核糖传感器。NAP2 和 NBAT-Ru 结合后，NBAT-Ru 构象的改变改变了 Ru 复合物和电极之间的距离，导致低 ECL 信号。通过将 5'-SH-(CH2)6-NBAT-Ru 自组装到 Au 电极上来制造信号开启脱氧核糖传感器。NAP2 的引入引发了适体结构域的构象变化，这导致 NBAT-Ru 的两个双螺旋茎的螺旋间堆叠。这种堆叠构成了“电接触”，它促进了电子空穴通过 NBAT-Ru 茎的传输，并产生了高 ECL 强度。两种脱氧核糖传感器都显示出高灵敏度和选择性。该生物传感器已成功应用于临床血浆检测。我们描述的方法代表了基于 DNA-TWJ 插入目标特殊结合域作为分子识别元件和用于制造生物传感器的不同固定类型的独特原理，对于蛋白质、金属离子、细菌和细胞的检测非常有希望。促进电子空穴通过 NBAT-Ru 茎的传输，并产生高 ECL 强度。两种脱氧核糖传感器都显示出高灵敏度和选择性。该生物传感器已成功应用于临床血浆检测。我们描述的方法代表了基于 DNA-TWJ 插入目标特殊结合域作为分子识别元件和用于制造生物传感器的不同固定类型的独特原理，对于蛋白质、金属离子、细菌和细胞的检测非常有希望。促进电子空穴通过 NBAT-Ru 茎的传输，并产生高 ECL 强度。两种脱氧核糖传感器都显示出高灵敏度和选择性。该生物传感器已成功应用于临床血浆检测。我们描述的方法代表了基于 DNA-TWJ 插入目标特殊结合域作为分子识别元件和用于制造生物传感器的不同固定类型的独特原理，对于蛋白质、金属离子、细菌和细胞的检测非常有希望。

更新日期：2020-03-01

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