Simultaneous recording of action potentials (APs) and neurotransmitter release is highly desirable in living neurons since it provides a complete framework of the physiological and pathological statuses of nerve cells. In this work, we proposed an approach coupling ultra-thin microelectrode array (MEA) with total internal reflection fluorescence microscopy (TIRFM), which served as a powerful platform to visualize both APs and vesicular exocytosis in a neuronal circuit model formed by neuron-like PC12 cells. Taking advantages of fluorescent false neurotransmitter (FFN), the transient neurotransmitter transport down an axon could be visualized with high spatial and temporal resolution. The real-time recording of APs burst and neurotransmitter release induced by hypoxia with MEA/TIRFM platform reveals the relevance of electrical and chemical activities in the neuronal model. The combination of the optical and electrical techniques enables mapping of neuron connectivity in an entire neuronal circuit, which may ultimately lead to deeper understanding of nervous system.

中文翻译：

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同时监测神经元样 PC12 细胞的动作电位和神经递质释放

同时记录动作电位 (AP) 和神经递质释放在活神经元中是非常理想的，因为它提供了神经细胞生理和病理状态的完整框架。在这项工作中，我们提出了一种将超薄微电极阵列 (MEA) 与全内反射荧光显微镜 (TIRFM) 相结合的方法，该方法是一个强大的平台，可在由类神经元形成的神经元电路模型中可视化 AP 和囊泡胞吐作用。 PC12 细胞。利用荧光假神经递质 (FFN)，可以以高空间和时间分辨率可视化沿轴突传输的瞬态神经递质。MEA/TIRFM 平台实时记录缺氧引起的 AP 爆发和神经递质释放，揭示了神经元模型中电和化学活动的相关性。光学和电学技术的结合能够绘制整个神经元回路中的神经元连接性，最终可能会导致对神经系统的更深入了解。