Short and medium fatty acids derived from either dietary sources, gut microbiota, and liver production might play a role in the modulation of metabolism and inflammation. The outcome of different autoimmune or inflammatory diseases could be related to microbiota composition and consequently fatty acids production. Their analytical detection, historically completed by GC, was herein investigated using a sensitive approach of LC-MS/MS with straightforward chemical derivatization, using 3-NPH, to the respective acylhydrazines. An isopropanol protein precipitation coupled to LC-MS/MS analysis allowed to separate and quantify butyric, valeric, hexanoic acid and their branched forms. The serum physiological ranges of short and medium chain fatty acids were determined in a heterogeneous healthy population (n=54) from 18-85 years findings a concentration of 935.6 ± 246.5 (butyric), 698.8 ± 204.7 (isobutyric), 62.9 ± 15.3 (valeric), 1155.0 ± 490.4 (isovaleric) and 468.7 ± 377.5 (hexanoic) ng/mL respectively (mean ± SD). As expected, the biological levels in human serum are reasonably wide-ranging depending on several factors such as body-weight, gut microbiome dysbiosis, gut permeability, cardiometabolic dysregulation, and diets.

来自饮食来源，肠道菌群和肝脏生产的短和中脂肪酸可能在代谢和炎症的调节中起作用。不同的自身免疫性疾病或炎性疾病的结局可能与微生物群的组成以及脂肪酸的产生有关。他们的分析检测历史上是由GC完成的，在这里使用LC-MS / MS的灵敏方法进行了研究，该方法采用3-NPH对各种酰基肼进行直接化学衍生化。异丙醇蛋白质沉淀与LC-MS / MS分析相结合，可以分离和量化丁酸，戊酸，己酸及其支链形式。在异质健康人群中测定短和中链脂肪酸的血清生理范围（n= 54）从18-85岁发现浓度分别为935.6±246.5（丁酸），698.8±204.7（异丁酸），62.9±15.3（戊酸），1155.0±490.4（等戊酸）和468.7±377.5（己酸）ng / mL （平均值±SD）。如预期的那样，人血清中的生物学水平在相当宽的范围内，具体取决于几个因素，例如体重，肠道微生物组功能失调，肠道通透性，心脏代谢失调和饮食。