Soils in the water-level-fluctuating zone (WLFZ) of Three Gorges Reservoir (TGR) inundated by water for different periods of time are confirmed to have disparate characteristics to mercury (Hg), and thus it is of great significance to further investigate microbial compositions and influencing factors. The objective of this study was to compare bacterial and archaeal richness, α-diversities and compositions, as well as affecting variables, especially Hg concentrations, among soils under different submergence time-SI (inundated soil), SS (semi-inundated soil), SN(non-inundated soil) and SSe (sediment)-based on high throughput sequencing. Results showed that sediment had significantly higher bacterial and archaeal richness and α-diversities than the other soil types. Anaerolinea and Aeromonas, as well as Altiarchaeales, Nitrosoarchaeum, and Methanosarta were dominant in SSe, while sharply decreasing in the other soil types, with significant difference among groups. An unclassified genus in SCG critically predominating in SI, SS and SN, drastically reduced in SSe, with extremely significant difference among groups. Bathyarchaeota and Nitrososphaera, both dominating in SSe, decreased dramatically and almost vanished in SI and SN. All the variables except pH posed a significant positive effect on bacterial and archaeal compositions in SSe, while opposite effect in the other three soil types. MeHg and THg concentrations had relatively weaker effects on microbial compositions comparing to variables like NH4+, CEC, OM and SO42+.

中文翻译：

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汞敏感储层中不同淹没时间下土壤中细菌和古细菌的组成及其影响因素。

在三峡水库（TGR）的水位波动区（WLFZ）中，水在不同的时间被淹没的土壤被证实具有与汞（Hg）不同的特性，因此，进一步研究微生物具有重要意义。组成和影响因素。这项研究的目的是比较不同淹没时间下土壤（SI）（淹没土壤），SS（半淹没土壤），土壤中细菌和古细菌的丰富度，α多样性和组成以及影响变量，尤其是汞浓度。基于高通量测序的SN（非淹没土壤）和SSe（沉积物）。结果表明，沉积物比其他土壤类型具有明显更高的细菌和古细菌丰富度和α多样性。厌氧菌和气单胞菌，以及高原古生物，硝基古生物，甲烷和甲烷菌在SSe中占主导地位，而在其他土壤类型中则急剧下降，各组之间差异显着。SCG中未分类的属在SI，SS和SN中至关重要，在SSe中则大大减少，各组之间的差异非常大。在SSe中占主导地位的Bathyarchaeota和Nitrososphaera在SI和SN中急剧下降，几乎消失。除pH以外的所有变量均对SSe中的细菌和古细菌组成具有显着的正效应，而在其他三种土壤类型中则具有相反的效应。与诸如NH4 +，CEC，OM和SO42 +等变量相比，MeHg和THg浓度对微生物组成的影响相对较弱。SCG中未分类的属在SI，SS和SN中至关重要，在SSe中则大大减少，各组之间的差异非常显着。在SSe中占主导地位的Bathyarchaeota和Nitrososphaera在SI和SN中急剧下降，几乎消失。除pH以外的所有变量均对SSe中的细菌和古细菌组成具有显着的正效应，而在其他三种土壤类型中则相反。与诸如NH4 +，CEC，OM和SO42 +等变量相比，MeHg和THg浓度对微生物组成的影响相对较弱。SCG中未分类的属在SI，SS和SN中至关重要，在SSe中则大大减少，各组之间的差异非常显着。在SSe中占主导地位的Bathyarchaeota和Nitrososphaera在SI和SN中急剧下降，几乎消失。除pH以外的所有变量均对SSe中的细菌和古细菌组成具有显着的正效应，而在其他三种土壤类型中则相反。与诸如NH4 +，CEC，OM和SO42 +等变量相比，MeHg和THg浓度对微生物组成的影响相对较弱。除pH以外的所有变量均对SSe中的细菌和古细菌组成具有显着的正效应，而在其他三种土壤类型中则相反。与诸如NH4 +，CEC，OM和SO42 +等变量相比，MeHg和THg浓度对微生物组成的影响相对较弱。除pH以外的所有变量均对SSe中的细菌和古细菌组成具有显着的正效应，而在其他三种土壤类型中则相反。与诸如NH4 +，CEC，OM和SO42 +等变量相比，MeHg和THg浓度对微生物组成的影响相对较弱。