The studied hypothesis is that glyphosate (GLY) can affect the oxidative balance in the hydrophilic intracellular medium in non-target Chlorella vulgaris cells. Analytical GLY (5 μM) and a commercial product (RUP) (5 μM) supplementation, did not affect the growth profile. Neither in latent (Lag) nor in exponential (Exp) phase of development, there were significant differences in the cellular abundance, evaluated as cell number, after the supplementation with GLY or RUP. The ascorbyl (A•) content was significantly increased in the presence of GLY or RUP, in Lag and Exp phase of growth. No changes were observed in stationary (St) phase after supplementation with either GLY or RUP. Ascorbate (AH-) content was decreased by 30% in Exp phase of development the presence of RUP. In St phase of the development both, the administration of either GLY or RUP decreased the antioxidant content by 34 and 37%, respectively. The supplementation with GLY and RUP lead to a significant 5- and 10-fold increase in Exp phase, respectively in the A•/AH-content ratio, assessed as a damage/protection ratio in the hydrophilic fraction of the cells, as compared to controls. Neither GLY nor RUP affected the ratio in cells in St phase of development. The data presented here showed experimental evidence that suggested that oxidative balance in the hydrophilic environment is affected by GLY, even at the low to medium concentrations currently used. The effect seems as reversible either because of the magnitude of the herbicide-dependent damage or the antioxidant activity activated.

中文翻译：

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评估暴露于草甘膦的小球藻亲水细胞环境中的氧化平衡。


研究的假设是，草甘膦 (GLY) 可以影响非目标小球藻细胞中亲水性细胞内介质的氧化平衡。分析 GLY (5 μM) 和商业产品 (RUP) (5 μM) 补充剂不会影响生长情况。补充 GLY 或 RUP 后，无论是在发育的潜伏期 (Lag) 还是指数期 (Exp)，细胞丰度（以细胞数量评估）均存在显着差异。在 GLY 或 RUP 存在下，在生长的 Lag 和 Exp 阶段，抗坏血酸 (A•) 含量显着增加。补充 GLY 或 RUP 后，稳定期 (St) 未观察到任何变化。在 RUP 存在的开发 Exp 阶段，抗坏血酸 (AH-) 含量降低了 30%。在开发的 St 阶段，施用 GLY 或 RUP 分别使抗氧化剂含量降低 34% 和 37%。与补充 GLY 和 RUP 相比，补充 GLY 和 RUP 导致 Exp 阶段的 A·/AH 含量比分别显着增加 5 倍和 10 倍，以细胞亲水部分的损伤/保护比来评估。控制。 GLY 和 RUP 都不影响发育 St 期细胞的比例。这里提供的数据显示的实验证据表明，亲水环境中的氧化平衡会受到 GLY 的影响，即使在目前使用的低至中等浓度下也是如此。由于除草剂依赖性损害的严重性或抗氧化活性的激活，这种效应似乎是可逆的。