Great progresses have been made to carry out Fenton oxidation under neutral or alkaline pH in which, nevertheless, organic acids and other acidic intermediates usually result in acidic Fenton effluent. To eliminate the classical neutralization step prior to biological treatment, acid-tolerant microbes were here screened and used for purification of acidic Fenton effluent to achieve pH increase and further COD (chemical oxygen demand) removal. The bacterial and fungal community diversity was analyzed before and after screening for acid-tolerant microbes. After screening the bacterial diversity sharply decreased while the fungal diversity at the genus level became richer, mainly including Phialemoniopsis (relative abundance 38.69%), Vanrija (20.08%), Hypocreaceae (18.44%) and Candida (14.74%). Acidic pH and residual H2O2 are the features of Fenton effluent; hence, effects of pH and H2O2 on the screened acid-tolerant microbes were investigated in the aspects of growth rate and oxygen uptake rate. The kinetic parameters, including YH-biomass yield coefficient; Kd-biomass decay coefficient; μm-specific maximum COD removal rate; Ks-half saturation constant for COD removal, of the acid-tolerant microbes using 1/5 YM (yeast extract and malt extract culture medium) as substrate at 25 °C were measured by respirometric methodology. In BAC (biological activated carbon) inoculated with acid-tolerant microbes to treat actual Fenton effluent, the average COD removal efficiency was 72% at HRT (hydraulic retention time) of 3 h and the effluent pH was above 6 after removing the dissolved CO2 by air stripping. This study will provide a basis for developing a new combined process including Fenton and biological oxidation without pH adjustment.

中文翻译：

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真菌财团在不添加碱的情况下无需预先中和的情况下，对酸性Fenton废水进行生物纯化：微生物筛选和性能。

在中性或碱性pH值下进行Fenton氧化已经取得了很大的进步，尽管如此，其中有机酸和其他酸性中间体通常会产生Fenton酸性流出物。为了消除生物处理之前的经典中和步骤，此处筛选了耐酸微生物，并将其用于纯化酸性Fenton废水以实现pH值增加和进一步去除COD（化学需氧量）。在筛选耐酸微生物之前和之后，对细菌和真菌群落多样性进行了分析。筛选后，细菌多样性急剧下降，而属水平的真菌多样性却变得更丰富，主要包括Ph虫（相对丰度38.69％），Vanrija（20.08％），Hypocreaceae（18.44％）和念珠菌（14.74％）。酸性pH和残留的H2O2是Fenton废水的特征。因此，从生长速率和吸氧率方面研究了pH和H2O2对筛选的耐酸微生物的影响。动力学参数包括YH-生物质产量系数；Kd生物量衰减系数；特定于μm的最大COD去除率；通过呼吸测定法测量了在25°C下使用1/5 YM（酵母提取物和麦芽提取物培养基）作为底物的耐酸微生物的COD去除的Ks-半饱和常数。在接种了耐酸微生物以处理实际的Fenton废水的BAC（生物活性炭）中，在HRT（水力停留时间）为3 h时，平均COD去除效率为72％，并且在通过H2O去除溶解的CO2后废水的pH值高于6。空气剥离。