In this study we report for the first time on a surface acoustic wave (SAW) sensor modified with a molecularly imprinted polymer (MIP) selectively recognizing the cerebral dopamine neurotrophic factor (CDNF) protein. CDNF-MIP as a synthetic recognition element was prepared by a simple electrochemical surface imprinting approach allowing its reliable interfacing with SAW sensor. The optimal thickness of the MIP layer as well as a suitable pretreatment method were adjusted in order to improve the recognition capacity and selectivity of the resulting CDNF-MIP sensor. The selectivity of the sensor was studied by the carefully designed competitive binding experiments, which revealed that the sensor can sense CNDF confidently in label-free manner starting from 0.1 pg/ml. We anticipate that the findings can be a premise for fabrication of cost-effective research or diagnostics tools in the field of neurodegenerative diseases.

在这项研究中，我们首次报道了用分子印迹聚合物（MIP）修饰的表面声波（SAW）传感器，该传感器选择性地识别了脑多巴胺神经营养因子（CDNF）蛋白。CDNF-MIP作为合成识别元件是通过简单的电化学表面压印方法制备的，从而使其能够与SAW传感器可靠地连接。调整了MIP层的最佳厚度以及合适的预处理方法，以提高所得CDNF-MIP传感器的识别能力和选择性。通过精心设计的竞争结合实验对传感器的选择性进行了研究，结果表明该传感器可以从0.1 pg / ml开始以无标记的方式自信地检测CNDF。