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Molecularly imprinted polymer-based SAW sensor for label-free detection of cerebral dopamine neurotrophic factor protein
Sensors and Actuators B: Chemical ( IF 8.0 ) Pub Date : 2020-01-18 , DOI: 10.1016/j.snb.2020.127708
Anna Kidakova , Roman Boroznjak , Jekaterina Reut , Andres Öpik , Mart Saarma , Vitali Syritski

In this study we report for the first time on a surface acoustic wave (SAW) sensor modified with a molecularly imprinted polymer (MIP) selectively recognizing the cerebral dopamine neurotrophic factor (CDNF) protein. CDNF-MIP as a synthetic recognition element was prepared by a simple electrochemical surface imprinting approach allowing its reliable interfacing with SAW sensor. The optimal thickness of the MIP layer as well as a suitable pretreatment method were adjusted in order to improve the recognition capacity and selectivity of the resulting CDNF-MIP sensor. The selectivity of the sensor was studied by the carefully designed competitive binding experiments, which revealed that the sensor can sense CNDF confidently in label-free manner starting from 0.1 pg/ml. We anticipate that the findings can be a premise for fabrication of cost-effective research or diagnostics tools in the field of neurodegenerative diseases.



中文翻译:

基于分子印迹聚合物的SAW传感器可无标记地检测脑多巴胺神经营养因子蛋白

在这项研究中,我们首次报道了用分子印迹聚合物(MIP)修饰的表面声波(SAW)传感器,该传感器选择性地识别了脑多巴胺神经营养因子(CDNF)蛋白。CDNF-MIP作为合成识别元件是通过简单的电化学表面压印方法制备的,从而使其能够与SAW传感器可靠地连接。调整了MIP层的最佳厚度以及合适的预处理方法,以提高所得CDNF-MIP传感器的识别能力和选择性。通过精心设计的竞争结合实验对传感器的选择性进行了研究,结果表明该传感器可以从0.1 pg / ml开始以无标记的方式自信地检测CNDF。

更新日期:2020-01-21
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