Ethidium Bromide (Eth-Br) is an intercalating agent commonly used in medical and biological laboratories as a DNA staining dye. Despite its popular use, aqueous solutions containing Eth-Br showed high toxicity, mutagenic capacity, and deactivate DNA transcription. In this study, the removal of Eth-Br from aqueous solutions by gamma irradiation has been fully investigated. Gamma irradiation was capable of achieving a near complete removal of Eth-Br in neutral and non-buffered aqueous solutions at an absorbed dose of 15 kGy. Various experimental conditions were studied and showed that the removal efficiency is not diminished. The addition of hydrogen peroxide (2 %) to the irradiated solutions reduced the D50 and D90 by 50 %. Modeling Eth-Br decomposition showed that the reaction followed pseudo first-order kinetics and reaches at least 90 % removal under all experimental conditions. TOC and HPLC measurements confirmed that Eth-Br is fully mineralized when the absorbed dose reaches 15 kGy. The biological activity of Eth-Br after irradiation treatment was investigated with synthetic DNA and natural DNA. The biological activity of Eth-Br was deactivated at an absorbed dose as low as 5 kGy. Toxicity measurement with E-coli bacteria also confirmed that the absorbed dose of 5 kGy was sufficient to remove Eth-Br toxicity.

中文翻译：

---

通过γ射线辐照分解DNA染色剂溴化乙锭：条件，动力学，副产物，生物活性和从废水中去除。

溴化乙锭（Eth-Br）是一种插入剂，通常在医学和生物实验室中用作DNA染色染料。尽管已广泛使用，但含有Eth-Br的水溶液仍显示出高毒性，诱变能力，并使DNA转录失活。在这项研究中，已经对通过伽马射线辐照从水溶液中去除Eth-Br进行了充分研究。γ射线能够以15 kGy的吸收剂量几乎完全去除中性和非缓冲水溶液中的Eth-Br。研究了各种实验条件，结果表明去除效率并未降低。向辐照溶液中添加过氧化氢（2％）可使D50和D90降低50％。Eth-Br分解模型表明，该反应遵循拟一级反应动力学，在所有实验条件下均达到至少90％的去除率。TOC和HPLC测量证实，当吸收剂量达到15 kGy时，Eth-Br完全矿化。用合成DNA和天然DNA研究了辐照处理后Eth-Br的生物学活性。Eth-Br的生物活性在低至5 kGy的吸收剂量下失活。用大肠杆菌进行的毒性测量还证实，吸收的5 kGy剂量足以消除Eth-Br毒性。Eth-Br的生物活性在低至5 kGy的吸收剂量下失活。用大肠杆菌进行的毒性测量还证实，吸收的5 kGy剂量足以消除Eth-Br毒性。Eth-Br的生物活性在低至5 kGy的吸收剂量下失活。用大肠杆菌进行的毒性测量还证实，吸收的5 kGy剂量足以消除Eth-Br毒性。