Multi-block glucans comprising permethylated and partially methylated blocks are compounds of interest. In order to monitor their formation by transglycosylation of corresponding starting glucans, a method has been developed and applied to model compounds. This method allows determining the average length of the blocks and the progress of incorporation of methyl blocks in partially methylated sequences with a random distribution. The method, comprising liquid chromatography mass spectrometry (LC-MS) and electrospray ionization collision-induced dissociation tandem mass spectrometry (ESI-CID-MSn) measurements of two types of peralkylated glucans representing derivatives of the target compounds, is comprehensively described and discussed. ESI-MSn allows looking into the sequences of oligomeric domains. In addition, transglycosylation is followed by attenuated total reflection FTIR and NMR spectroscopy. Graphical abstract.

中文翻译：

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1,4-D-葡聚糖嵌段共聚物：合成和全面的结构表征。

包含全甲基化和部分甲基化的嵌段的多嵌段葡聚糖是感兴趣的化合物。为了通过相应的起始葡聚糖的转糖基化来监测其形成，已经开发了一种方法并将其应用于模型化合物。该方法允许确定嵌段的平均长度以及甲基嵌段在具有随机分布的部分甲基化序列中掺入的进展。对包括代表目标化合物衍生物的两种类型的全烷基化葡聚糖的液相色谱质谱（LC-MS）和电喷雾电离碰撞诱导解离串联质谱（ESI-CID-MSn）测量进行了全面描述和讨论。ESI-MSn允许研究寡聚域的序列。此外，转糖基化之后是衰减全反射FTIR和NMR光谱。图形概要。