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Regrowth of zebrafish caudal fin regeneration is determined by the amputated length.
Scientific Reports ( IF 3.8 ) Pub Date : 2020-01-20 , DOI: 10.1038/s41598-020-57533-6
Toshiaki Uemoto 1 , Gembu Abe 1 , Koji Tamura 1
Affiliation  

Fish have a high ability to regenerate fins, including the caudal fin. After caudal fin amputation, original bi-lobed morphology is reconstructed during its rapid regrowth. It is still controversial whether positional memory in the blastema cells regulates reconstruction of fin morphology as in amphibian limb regeneration, in which limb blastema cells located at the same proximal-distal level have the same positional identity. We investigated growth period and growth rate in zebrafish caudal fin regeneration. We found that both the growth period and growth rate differed for fin rays that were amputated at the same proximal-distal level, indicating that it takes different periods of time for fin rays to restore their original lengths after straight amputation. We also show that more proximal amputation takes longer period to reconstruct the original morphology/size than more distal amputation. Statistical analysis suggested that both the growth period/rate are determined by amputated length (depth) regardless of the fin ray identity along dorsal-ventral axis. In addition, we suggest the possibility that the structural/physical condition such as width of the fin ray at the amputation site (niche at the stump) may determine the growth period/rate.

中文翻译:

斑马鱼尾鳍的再生长取决于截肢的长度。

鱼具有很高的再生鳍的能力,包括尾鳍。尾鳍截肢后,原始的双叶形态在其快速长生期间得以重建。如在两栖动物肢体再生中一样,母细胞瘤细胞中的位置记忆是否调节鳍形态的重建仍存在争议,在两栖动物肢体再生中,位于相同近端-远端水平的肢体母细胞具有相同的位置同一性。我们调查了斑马鱼尾鳍再生的生长期和生长速率。我们发现,在相同的近端至远端水平上被截肢的鳍条的生长期和增长率均不同,这表明鳍条在直截肢后恢复其原始长度需要花费不同的时间。我们还显示,与更多的远端截肢相比,更多的近端截肢需要更长的时间来重建原始形态/大小。统计分析表明,无论长短/深度长短都取决于截肢的长度(深度),而与背腹轴上的鳍片身份无关。另外,我们提出了这样的可能性,即截肢部位的鳍条宽度(树桩上的生态位)等结构/物理条件可能决定生长时期/速率。
更新日期:2020-01-21
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