To examine the influence on postoperative endothelial cell density (ECD) caused by preoperative temperature change from 4 °C to room temperature in donor corneas for Descemet stripping automated endothelial keratoplasty (DSAEK). This retrospective, case-controlled comparison of 100 eyes that underwent DSAEK using imported donor corneas transferred from an overseas eye bank (SightLife, Seattle, WA, USA). Fifty donor corneas experienced temperature reversal for ECD measurement (TR group), and postoperative outcomes were compared with 50 disease-matched cases that did not experience temperature changes before DSAEK (NTR group). The main outcome measure was endothelial cell loss and reduction rate of ECD at 1, 3, and 6 months following DSAEK. ECD at 3 months following DSAEK was significantly less in the TR group (1458 ± 494/mm2) than in the NTR group (1696 ± 374/mm2; P = 0.014), though ECD at 6 months was not significantly less in the TR group. The reduction rate of ECD at 3 months was greater in the TR group (42.3% ± 17.2%) than in the NTR group (35.7% ± 14.2%; P = 0.044), though reduction rate of ECD at 6 months was not significantly less in the TR group. We found that preoperative temperature change in donor corneas may adversely affect ECD following DSAEK.

中文翻译：

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供体角膜的温度变化对内皮移植后术后内皮细胞密度的影响。

为了检查Descemet剥离自动内皮角膜移植术（DSAEK）在供体角膜中由术前温度从4°C到室温的变化对手术后内皮细胞密度（ECD）的影响。这项回顾性病例对照研究比较了使用从国外眼库（SightLife，西雅图，华盛顿州，美国）转移过来的进口供体角膜对DSAEK进行的100只眼睛的情况。五十个供体角膜经历了ECD测量的温度逆转（TR组），并将术后结果与50例在DSAEK之前没有经历温度变化的疾病匹配病例（NTR组）进行了比较。主要结果指标是DSAEK后1、3和6个月时内皮细胞的丢失和ECD的减少率。DSAEK治疗后3个月的ECD明显低于NTR组（1696±374 / mm2; P = 0.014），尽管DSAEK后3个月的ECD明显低于TR组。 。TR组（32.3％±17.2％）在3个月时的ECD降低率高于NTR组（35.7％±14.2％; P = 0.044），尽管6个月时ECD的降低率并没有明显降低在TR小组中。我们发现DSAEK后，供体角膜的术前温度变化可能会对ECD产生不利影响。