The role of long noncoding RNA ZEB1 antisense 1 (lncRNA ZEB1-AS1) in carotid artery atherosclerosis remains barely explored. The viability and apoptosis of HCtAEC cells were measured by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Caspase-3 activity detection assay and flow cytometry. The oxidative stress status and inflammation of THP-1 cells were detected by oxidative stress indicator detection kit and Enzyme-linked immunosorbent assay (ELISA). The abundance of ZEB1-AS1, miR-942 and high-mobility group box 1 (HMGB1) was measured by quantitative real time polymerase chain reaction (qRT-PCR). The targets of ZEB1-AS1 and miR-942 in HCtAEC and THP-1 cells were predicted by DIANA tool, and the combination was confirmed by dual-luciferase reporter assay, RNA immunoprecipitation (RIP) assay and RNA-pull down assay. Western blot was conducted to examine the protein expression of HMGB1. ZEB1-AS1 promoted ox-LDL-mediated injury in HCtAEC and THP-1 cells. MiR-942 was a direct target of ZEB1-AS1, and it was negatively modulated by ZEB1-AS1 in HCtAEC and THP-1 cells. HMGB1 could bind to miR-942, and it was regulated by ZEB1-AS1/miR-942 axis in HCtAEC and THP-1 cells. HMGB1 overexpression or miR-942 depletion reversed the inhibitory effects of ZEB1-AS1 intervention on the injury and apoptosis of HCtAEC cells and the oxidative stress and inflammation of THP-1 cells. LncRNA ZEB1-AS1 contributed to ox-LDL-mediated injury and apoptosis of HCtAEC cells and the oxidative stress and inflammation of THP-1 cells through up-regulating HMGB1 via sponging miR-942. ZEB1-AS1/miR-942/HMGB1 axis might provide a new direction to treatment carotid artery atherosclerosis.

中文翻译：

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LncRNA ZEB1-AS1 通过 miR-942/HMGB1 信号传导促进 ox-LDL 诱导的 HCtAEC 细胞损伤以及 THP-1 细胞的氧化应激和炎症事件

长链非编码 RNA ZEB1 反义 1 (lncRNA ZEB1-AS1) 在颈动脉粥样硬化中的作用尚未被探索。采用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑(MTT)、Caspase-3活性检测法和流式细胞术检测HCtAEC细胞的活力和凋亡。采用氧化应激指标检测试剂盒和酶联免疫吸附法（ELISA）检测THP-1细胞的氧化应激状态和炎症反应。通过定量实时聚合酶链反应 (qRT-PCR) 测量 ZEB1-AS1、miR-942 和高迁移率组盒 1 (HMGB1) 的丰度。通过DIANA工具预测ZEB1-AS1和miR-942在HCtAEC和THP-1细胞中的靶点，并通过双荧光素酶报告基因测定、RNA免疫沉淀（RIP）测定和RNA-pull down测定确认组合。进行Western blot检测HMGB1的蛋白表达。ZEB1-AS1 促进 ox-LDL 介导的 HCtAEC 和 THP-1 细胞损伤。MiR-942是ZEB1-AS1的直接靶标，在HCtAEC和THP-1细胞中它受到ZEB1-AS1的负调节。HMGB1可以与miR-942结合，并且在HCtAEC和THP-1细胞中受到ZEB1-AS1/miR-942轴的调节。HMGB1过表达或miR-942缺失逆转了ZEB1-AS1干预对HCtAEC细胞损伤和凋亡以及THP-1细胞氧化应激和炎症的抑制作用。LncRNA ZEB1-AS1 通过海绵 miR-942 上调 HMGB1，导致 ox-LDL 介导的 HCtAEC 细胞损伤和凋亡，以及 THP-1 细胞的氧化应激和炎症。ZEB1-AS1/miR-942/HMGB1轴可能为治疗颈动脉粥样硬化提供新方向。