Abstract β-Thalassaemia is an inherited blood disorder with serious complications. Combining the unique advantages of DNA and magnetoelastic (ME) materials, an ME DNA-biosensor was proposed that can wirelessly detect a target DNA sequence (tDNA) that is a 4-bp deletion in codon 41/42 (-TTCT) in the β-globin gene causing β-thalassaemia. The thiolated capture probe (CP) was covalently immobilized on the surface of the gold-plated ME chip and then hybridized to tDNA, and AuNPs modified with thiolated signal probe DNA (sDNA-AuNPs) serve as the signal amplifier and the direct signal indicator, enabling label-free detection. The specific hybridization process of sDNA-AuNPs to tDNA increased the surface load mass and decreased the resonance frequency of the DNA-biosensor. The resonance frequency shift of the DNA-biosensor was linear to the logarithmic concentration of tDNA in the range of 1.0 × 10−8 M to 1.0 × 10−12 M, with a detection limit (LOD) of 0.571 pM (S/N = 3) and sensitivity of 72.7 Hz/nM. The ME DNA-biosensor exhibits excellent selectivity and stability for the detection of the mutated DNA (4-bp deletion in codon 41/42) causing β-thalassaemia, suggesting this is a promising method for the clinical diagnosis of β-thalassaemia.

中文翻译：

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AuNPs 放大的无线磁弹性 DNA 生物传感器，用于检测导致 β-地贫的常见突变 DNA

摘要 β-地贫是一种具有严重并发症的遗传性血液病。结合DNA和磁弹性（ME）材料的独特优势，提出了一种ME DNA生物传感器，可以无线检测β中密码子41/42（-TTCT）中4-bp缺失的靶DNA序列（tDNA）。 -珠蛋白基因导致β-地贫。将硫醇化捕获探针 (CP) 共价固定在镀金 ME 芯片表面，然后与 tDNA 杂交，用硫醇化信号探针 DNA (sDNA-AuNPs) 修饰的 AuNPs 作为信号放大器和直接信号指示剂，启用无标记检测。sDNA-AuNPs 与 tDNA 的特异性杂交过程增加了表面负载质量并降低了 DNA 生物传感器的共振频率。DNA-生物传感器的共振频移与 tDNA 在 1.0 × 10−8 M 到 1.0 × 10−12 M 范围内的对数浓度呈线性关系，检测限 (LOD) 为 0.571 pM (S/N = 3) 灵敏度为 72.7 Hz/nM。ME DNA-生物传感器在检测引起β-地贫的突变DNA（密码子41/42中4-bp缺失）方面表现出优异的选择性和稳定性，表明这是一种有前途的β-地贫临床诊断方法。