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Determination of n-alkane partitioning within phosphatidylethanolamine Lα/HII phases.
Biochimica et Biophysica Acta (BBA) - Biomembranes ( IF 2.8 ) Pub Date : 2020-01-20 , DOI: 10.1016/j.bbamem.2020.183201
Adrian Paz Ramos 1 , Mahmoudreza Doroudgar 1 , Michel Lafleur 1
Affiliation  

Alkanes are known to promote the fluid lamellar (Lα)-to-inverted hexagonal (HII) phase transition of different phospholipids. In this work, we studied the interaction of decane and tetradecane with self-assemblies formed of 1-palmitoyl-2-oleoyl-sn-glycero-phosphoethanolamine (POPE), using sequential 2H and 31P solid-state NMR spectroscopy. This technique allowed calculating the partitioning constant of the alkanes between the Lα and HII phases of POPE. Our results show that both alkanes are preferentially distributed in the HII phase compared to the Lα phase. In the HII phase, both alkanes display a very high conformational disorder, consistent with their location in the intercylinder voids. This preferential partitioning in the HII phase is more pronounced for tetradecane than for decane. This finding is proposed to be associated with a less energetically favored insertion (limited solubility) of tetradecane in the lamellar phase. This proposition is supported by the observation that tetradecane experiences more conformational freedom than its shorter analog in POPE bilayers, suggesting that it is located, on average, closer to the middle of the bilayers. It is also established that the increase in size of the intercylinder voids, by the addition of 1-palmitoyl-2-oleoyl-sn-glycero-phosphocholine (POPC) in the HII cylinders, enhances the partitioning of decane in the HII phase compared to the Lα phase. These findings propose handles to modulate the balance of the relative Lα/HII phase stability.

中文翻译:

磷脂酰乙醇胺Lα/ HII相中正构烷烃分配的测定。

已知烷烃可促进不同磷脂的流体层状(Lα)转变为六角形(HII)转化。在这项工作中,我们使用顺序2H和31P固态NMR光谱研究了癸烷和十四烷与1-棕榈酰基-2-油酰基-sn-甘油-磷酸乙醇胺(POPE)形成的自组装体的相互作用。该技术允许计算烷烃在POPE的Lα和HII相之间的分配常数。我们的结果表明,与Lα相相比,两种烷烃均优先分布在HII相中。在HII相中,两种烷烃均显示出很高的构象紊乱,这与它们在圆柱间空隙中的位置一致。在HII相中,这种优先分配对十四烷比对癸烷更为明显。提出该发现与十四烷在层状相中在能量上不太有利的插入(有限的溶解度)有关。这一观点得到了以下观察结果的支持:在POPE双层中,十四烷比其较短的类似物经历更多的构象自由,这表明它通常位于更靠近双层中间的位置。还已经确定,与HII汽缸相比,通过在HII汽缸中添加1-棕榈酰基-2-油酰基-sn-甘油-磷酸胆碱(POPC)来增加汽缸间空隙的尺寸,可以提高癸烷在HII相中的分配Lα相。这些发现提出了调节相对Lα/ HII相稳定性平衡的方法。这一观点得到了以下观察结果的支持:在POPE双层中,十四烷比其较短的类似物经历更多的构象自由,这表明它通常位于更靠近双层中间的位置。还已经确定,与HII汽缸相比,通过在HII汽缸中添加1-棕榈酰基-2-油酰基-sn-甘油-磷酸胆碱(POPC)来增加汽缸间空隙的尺寸,可以提高癸烷在HII相中的分配Lα相。这些发现提出了调节相对Lα/ HII相稳定性平衡的方法。这一观点得到了以下观察结果的支持:在POPE双层中,十四烷比其较短的类似物经历更多的构象自由,这表明它通常位于更靠近双层中间的位置。还已经确定,与HII汽缸相比,通过在HII汽缸中添加1-棕榈酰基-2-油酰基-sn-甘油-磷酸胆碱(POPC)来增加汽缸间空隙的尺寸,可以提高癸烷在HII相中的分配Lα相。这些发现提出了调节相对Lα/ HII相稳定性平衡的方法。通过在HII钢瓶中添加1-棕榈酰基-2-油酰基-sn-甘油磷酸胆碱(POPC),与Lα相相比,可增强癸烷在HII相中的分配。这些发现提出了调节相对Lα/ HII相稳定性平衡的方法。通过在HII钢瓶中添加1-棕榈酰基-2-油酰基-sn-甘油磷酸胆碱(POPC),与Lα相相比,可增强癸烷在HII相中的分配。这些发现提出了调节相对Lα/ HII相稳定性平衡的方法。
更新日期:2020-01-21
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