Abstract Due to the weak electrochemical behavior of 17α – ethinylestradiol, it is impossible to determine small amounts of 17α – ethinylestradiol (EE2) at the surface of conventional electrodes. Therefore, the designing and fabrication of electrochemical sensors with high performance are important. In this context, we developed a highly sensitive electrochemical sensor for EE2 detection based on multiwall carbon nanotubes and magnetic nanoparticles functionalized with tannic acid and Au nanoparticles (Au/Fe3O4@TA/MWNT). The hybrid nanocomposite deposited onto the glassy carbon electrode (GCE) showed excellent electrochemical properties so that the oxidation current of EE2 is dramatically increased by 28 times compared to GCE. The proposed novel sensor showed a good LOD and linear range (3.3 nM and 0.01–120 μM, respectively) for the quantification of EE2. In addition, the proposed sensor showed good selectivity for EE2 detection in the presence of several common interfering compounds. The high sensitivity and selectivity of the development sensor are related to the synergistic effect of the Fe3O4, Au nanoparticles (AuNPs), and MWNT used in the nanocomposite structure. The obtained results of real sample analysis by EE2 sensor suggest the sensor can be used as a potential tool for the determination of EE2 in different real samples.

中文翻译：

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使用 Au/Fe3O4@TA/MWNT/GCE 传感器对生物样品中的 17α – 炔雌醇进行电化学定量

摘要 由于 17α-炔雌醇的电化学行为较弱，无法在常规电极表面测定少量 17α-炔雌醇 (EE2)。因此，高性能电化学传感器的设计和制造非常重要。在此背景下，我们开发了一种基于多壁碳纳米管和用单宁酸和 Au 纳米颗粒（Au/Fe3O4@TA/MWNT）功能化的磁性纳米颗粒的高灵敏度电化学传感器，用于 EE2 检测。沉积在玻璃碳电极（GCE）上的混合纳米复合材料显示出优异的电化学性能，因此与 GCE 相比，EE2 的氧化电流显着增加了 28 倍。所提出的新型传感器显示出良好的 LOD 和线性范围（3.3 nM 和 0.01-120 μM，分别）用于 EE2 的量化。此外，所提出的传感器在存在几种常见干扰化合物的情况下对 EE2 检测显示出良好的选择性。显影传感器的高灵敏度和选择性与纳米复合结构中使用的 Fe3O4、Au 纳米粒子 (AuNPs) 和 MWNT 的协同效应有关。EE2 传感器对实际样品的分析结果表明，该传感器可作为测定不同实际样品中 EE2 的潜在工具。