A novel class of fluorescent “turn on” probe based on the fluorescence resonance energy transfer (FRET) approach was designed, and successfully synthesized for the detection of hydrogen sulfide (H₂S) both in vitro and in vivo. This new H₂S responsive fluorescent probe was developed based on the basis of the dabsyl and fluorescein FRET system. In the presence of H₂S, a fluorescence enhancement was markedly observed mainly due to the inhibition of the FRET process. This probe could quantitatively measure the level of H₂S with the detection limit of 0.02 μM. Cell imaging results demonstrated the potential applicability of a dabsylfluorescein probe for H₂S detection in living cells.

一类新的基于荧光共振能量转移荧光探针“上转”（FRET）方法被设计的，用于检测的硫化氢（H成功地合成₂ S）二者在体外和体内。这种新的H ₂ S响应荧光探针是基于dabsyl和荧光素FRET系统开发的。在H ₂ S的存在下，主要由于抑制了FRET过程，显着观察到荧光增强。该探针可以定量检测H ₂ S的水平，检测限为0.02μM。细胞成像结果证明了dabsylfluorescein探针对H ₂的潜在适用性活细胞中的S检测。