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Protein-stabilized gold nanoclusters for PDT: ROS and singlet oxygen generation.
Journal of Photochemistry and Photobiology B: Biology ( IF 3.9 ) Pub Date : 2020-01-20 , DOI: 10.1016/j.jphotobiol.2020.111802
Vilius Poderys 1 , Greta Jarockyte 1 , Saulius Bagdonas 2 , Vitalijus Karabanovas 3 , Ricardas Rotomskis 4
Affiliation  

Suitable properties as well as eco-friendly synthesis of photoluminescent Au nanoclusters (NCs) make them promising compounds for biomedical diagnostics and visualization applications. However, the potential photochemical activity of such agents on cancerous cells is largely unknown. The nanoclusters (BSA-Au NCs) were synthetized in the presence of BSA (an average hydrodynamic diameter was about 9.4 nm, while the size of the metal cluster was <1.3 nm according to atomic force microscopy measurements) and possessed a broad photoluminescence band at 680 nm in buffered (pH 7.2) aqueous medium. The photochemical activity was studied by adding two fluorescent probes (dihydrorhodamine or Singlet Oxygen Sensor Green) for detection of reactive oxygen species in samples irradiated at 405 nm to minimize direct excitation of the probes. The photoluminescence measurements evidenced the capability of BSA-Au NCs to generate reactive oxygen species upon light exposure, while the observed sensitivity of the photoluminescence properties might be used to indicate photooxidative processes in the medium. The viability test performed on breast cancer cells after incubation with BSA-Au NCs and subsequent irradiation revealed notable difference in induced phototoxicity between two cell lines, which was not the case after the corresponding treatment using the photosensitizer chlorin e6.

中文翻译:

用于PDT的蛋白质稳定的金纳米簇:ROS和单线态氧的产生。

合适的性能以及光致发光的金纳米簇(NCs)的环保合成使其成为生物医学诊断和可视化应用的有前途的化合物。然而,这种试剂对癌细胞的潜在光化学活性在很大程度上是未知的。纳米团簇(BSA-Au NCs)是在BSA存在下合成的(根据原子力显微镜测量,平均流体动力学直径约为9.4 nm,而金属团簇的大小<1.3 nm),并具有较宽的光致发光谱带。缓冲(pH 7.2)水性介质中的680 nm。通过添加两个荧光探针(二氢罗丹明或单线态氧传感器绿色)来研究光化学活性,以检测在405 nm照射的样品中的活性氧,以最大程度地减少探针的直接激发。光致发光测量结果证明了BSA-Au NCs在曝光时具有产生活性氧的能力,而观察到的光致发光特性敏感性可用于指示介质中的光氧化过程。用BSA-Au NCs孵育后,对乳腺癌细胞进行的生存力测试表明,在两种细胞系之间诱导的光毒性存在显着差异,而在使用光敏剂二氢卟酚e6进行相应处理后,情况并非如此。
更新日期:2020-01-21
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