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Cloning and functional characterization of an elovl4-like gene involved in the biosynthesis of long-chain polyunsaturated fatty acids in the swimming crab Portunus trituberculatus.
Comparative Biochemistry and Physiology B: Biochemistry & Molecular Biology ( IF 1.9 ) Pub Date : 2020-01-17 , DOI: 10.1016/j.cbpb.2020.110408 Peng Sun 1 , Qicun Zhou 1 , Óscar Monroig 2 , Juan Carlos Navarro 2 , Min Jin 3 , Ye Yuan 3 , Xuexi Wang 3 , Lefei Jiao 3
Comparative Biochemistry and Physiology B: Biochemistry & Molecular Biology ( IF 1.9 ) Pub Date : 2020-01-17 , DOI: 10.1016/j.cbpb.2020.110408 Peng Sun 1 , Qicun Zhou 1 , Óscar Monroig 2 , Juan Carlos Navarro 2 , Min Jin 3 , Ye Yuan 3 , Xuexi Wang 3 , Lefei Jiao 3
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Elongation of very long-chain fatty acid 4 (Elovl4) proteins participate in the biosynthesis of long-chain and very long-chain polyunsaturated fatty acids (LC-PUFA and VLC-PUFA). In the present study, an elovl4 cDNA was cloned from the swimming crab Portunus trituberculatus by PCR techniques and functionally characterized using recombinant expression in yeast Saccharomyces cerevisiae. The elovl4 cDNA sequence contained an open reading frame of 1038 base pairs, encoding a protein of 346 amino acids. The elovl4 has typical Elovl structures, with transmembrane domains (6) and a histidine box. The elovl4 was expressed in various tissues analyzed, with the highest expression found in intestine and hepatopancreas, followed by stomach and eyestalk. The functional characterization of Elovl4 yeast showed that the P. trituberculatus Elovl4 can elongate C18-22 polyunsaturated fatty acids (PUFA), reaching in some cases products of C24 and C26. Along its ability to elongate PUFA, the P. trituberculatus Elovl4 was also efficient in the elongation of saturated fatty acids, with 28:0 and 30:0 being prominent elongation products. These results provide insight into the LC-PUFA biosynthetic capability of commercially important species of crustaceans.
中文翻译：
游泳蟹Portunus trituberculatus中长链多不饱和脂肪酸生物合成中涉及的lovel4类基因的克隆和功能表征。
极长链脂肪酸4（Elovl4）蛋白质的伸长参与长链和极长链多不饱和脂肪酸（LC-PUFA和VLC-PUFA）的生物合成。在本研究中，通过PCR技术从游泳蟹Portunus trituberculatus中克隆出了lovo4 cDNA，并通过在酿酒酵母中的重组表达对其功能进行了表征。elov14 cDNA序列包含一个1038个碱基对的开放阅读框，编码346个氨基酸的蛋白质。elovl4具有典型的Elovl结构，具有跨膜结构域（6）和组氨酸盒。elovl4在所分析的各种组织中表达，在肠道和肝胰腺中的表达最高，其次是胃和眼睛。Elovl4酵母的功能表征显示P. trituberculatus Elovl4可以延长C18-22多不饱和脂肪酸（PUFA），在某些情况下可以达到C24和C26的产物。由于其延长PUFA的能力，P。trituberculatus Elovl4在饱和脂肪酸的延伸方面也很有效，其中28：0和30：0是突出的延伸产物。这些结果为商业上重要的甲壳类物种的LC-PUFA生物合成能力提供了见识。
更新日期：2020-01-21
中文翻译：
游泳蟹Portunus trituberculatus中长链多不饱和脂肪酸生物合成中涉及的lovel4类基因的克隆和功能表征。
极长链脂肪酸4（Elovl4）蛋白质的伸长参与长链和极长链多不饱和脂肪酸（LC-PUFA和VLC-PUFA）的生物合成。在本研究中，通过PCR技术从游泳蟹Portunus trituberculatus中克隆出了lovo4 cDNA，并通过在酿酒酵母中的重组表达对其功能进行了表征。elov14 cDNA序列包含一个1038个碱基对的开放阅读框，编码346个氨基酸的蛋白质。elovl4具有典型的Elovl结构，具有跨膜结构域（6）和组氨酸盒。elovl4在所分析的各种组织中表达，在肠道和肝胰腺中的表达最高，其次是胃和眼睛。Elovl4酵母的功能表征显示P. trituberculatus Elovl4可以延长C18-22多不饱和脂肪酸（PUFA），在某些情况下可以达到C24和C26的产物。由于其延长PUFA的能力，P。trituberculatus Elovl4在饱和脂肪酸的延伸方面也很有效，其中28：0和30：0是突出的延伸产物。这些结果为商业上重要的甲壳类物种的LC-PUFA生物合成能力提供了见识。
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