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Microplastics and copper effects on the neotropical teleost Prochilodus lineatus: Is there any interaction?
Comparative Biochemistry and Physiology A: Molecular & Integrative Physiology ( IF 2.1 ) Pub Date : 2020-01-18 , DOI: 10.1016/j.cbpa.2020.110659
Jéssica Fernanda Bernardes Roda 1 , Mariana Machado Lauer 1 , Wagner Ezequiel Risso 1 , Claudia Bueno Dos Reis Martinez 1
Affiliation  

Microplastics (MP) are emerging contaminants widely found in aquatic ecosystems. In addition to MP toxicity itself, there is increasing concern about the MP adsorption capacity and the interactive effects with other contaminants, such as copper. The objective of this research was to investigate the effects of polyethylene microplastic and its association with copper (Cu) in genotoxic, biochemical, and physiological biomarkers of the neotropical teleost Prochilodus lineatus. Fish were exposed for 24 and 96 h to MP (20 μg L-1) and Cu (10 μg L-1) and MP + Cu. The results showed that MP and Cu, both isolated and in combination, promoted DNA damage in erythrocytes (96 h) and liver cells (24 and 96 h) indicating that MP and Cu are genotoxic. Fish exposed only to Cu (96 h) showed a decrease in lipid peroxidation in the liver despite of the decrease in glutathione content, indicating the efficiency of other antioxidant defenses. Brain acetylcholinesterase was inhibited in the animals from all the treatments. Although MP did not influence on Cu accumulation in tissues, decreased plasma Na+ and Ca2+ (24 h) occurred after the exposure to MP and Cu, isolated and combined. Exposure to MP and MP + Cu resulted in decreased activity of Ca2+-ATPase (24 h). Taken altogether, these results showed that MP and Cu depicted genotoxic, neurotoxic, and physiological effects on P. lineatus, both alone and combined. An interaction between Cu and MP was observed in plasma Ca2+, where the combination of both contaminants caused a greater effect than the contaminants alone.

中文翻译:

微塑料和铜对新近性硬骨鱼类Prochilodus lineatus的影响:有相互作用吗?

微塑料(MP)是在水生生态系统中广泛发现的新兴污染物。除了MP本身的毒性外,人们对MP的吸附能力以及与其他污染物(例如铜)的相互作用也越来越关注。这项研究的目的是调查聚乙烯微塑料及其与铜(Cu)的关系在新热带硬骨Prochilodus lineatus的遗传毒性,生化和生理生物标记中的作用。将鱼暴露于MP(20μgL-1)和Cu(10μgL-1)和MP + Cu中24和96 h。结果表明,MP和Cu既分离又结合在一起,可促进红细胞(96 h)和肝细胞(24和96 h)的DNA损伤,表明MP和Cu具有遗传毒性。尽管谷胱甘肽含量下降,但仅暴露于铜的鱼(96小时)肝脏中的脂质过氧化作用降低,表明其他抗氧化剂防御措施的有效性。在所有处理中,动物体内的脑乙酰胆碱酯酶均被抑制。尽管MP不会影响组织中Cu的积累,但在分离和合并MP和Cu后,血浆Na +和Ca2 +降低(24 h)。暴露于MP和MP + Cu导致Ca2 + -ATPase活性降低(24小时)。总而言之,这些结果表明,MP和Cu分别或联合表现出对P. lineatus的遗传毒性,神经毒性和生理作用。在血浆Ca2 +中观察到Cu与MP之间的相互作用,其中两种污染物的组合所产生的影响要大于单独的污染物。表明其他抗氧化剂防御的效率。在所有处理中,动物体内的脑乙酰胆碱酯酶均被抑制。尽管MP不会影响组织中Cu的积累,但分离并结合后,血浆Na +和Ca2 +的含量下降(24小时)。暴露于MP和MP + Cu导致Ca2 + -ATPase活性降低(24小时)。总而言之,这些结果表明,MP和Cu分别或联合表现出对P. lineatus的遗传毒性,神经毒性和生理作用。在血浆Ca2 +中观察到Cu与MP之间的相互作用,其中两种污染物的组合所产生的影响要大于单独的污染物。表明其他抗氧化剂防御的效率。在所有处理中,动物体内的脑乙酰胆碱酯酶均被抑制。尽管MP不会影响组织中Cu的积累,但在分离和合并MP和Cu后,血浆Na +和Ca2 +降低(24 h)。暴露于MP和MP + Cu导致Ca2 + -ATPase活性降低(24小时)。总而言之,这些结果表明,MP和Cu分别或联合表现出对P. lineatus的遗传毒性,神经毒性和生理作用。在血浆Ca2 +中观察到Cu与MP之间的相互作用,其中两种污染物的组合所产生的影响要大于单独的污染物。尽管MP不会影响组织中Cu的积累,但在分离和合并MP和Cu后,血浆Na +和Ca2 +降低(24 h)。暴露于MP和MP + Cu导致Ca2 + -ATPase活性降低(24小时)。总而言之,这些结果表明,MP和Cu分别或联合表现出对P. lineatus的遗传毒性,神经毒性和生理作用。在血浆Ca2 +中观察到Cu与MP之间的相互作用,其中两种污染物的组合所产生的影响要大于单独的污染物。尽管MP不会影响组织中Cu的积累,但在分离和合并MP和Cu后,血浆Na +和Ca2 +降低(24 h)。暴露于MP和MP + Cu导致Ca2 + -ATPase活性降低(24小时)。总而言之,这些结果表明,MP和Cu分别或联合表现出对P. lineatus的遗传毒性,神经毒性和生理作用。在血浆Ca2 +中观察到Cu与MP之间的相互作用,其中两种污染物的组合所产生的影响要大于单独的污染物。和对线虫的生理影响,无论是单独还是联合使用。在血浆Ca2 +中观察到Cu与MP之间的相互作用,其中两种污染物的组合所产生的影响要大于单独的污染物。和对线虫的生理影响,无论是单独还是联合使用。在血浆Ca2 +中观察到Cu与MP之间的相互作用,其中两种污染物的组合所产生的影响要大于单独的污染物。
更新日期:2020-01-21
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