It was well known that a critical process of oogenesis in the female mammalian was the entry of mitotic oogonia into meiosis. Early studies from model animal mice suggested that the retinoic acid (RA) response signal protein STRA8 (stimulated by retinoic acid gene 8) and the meiosis-specific chromosomal behavior marker protein SCP3 (Synaptonemal Complex Protein 3) were two crucial molecular markers during meiosis. The expression of STRA8 and SCP3 at different stages in rat ovaries was investigated by immunohistochemistry, qRT-PCR and Western Blot. Immunohistochemistry results showed that STRA8 and SCP3 were mainly expressed in embryonic stage. And STRA8 was expressed in the cytoplasm and nucleus of the ovaries after birth. qRT-PCR and Western Blot results showed that the mRNA and protein levels of STRA8 and SCP3 were expressed in embryonic stage. The expression of STRA8 and SCP3 indicated germ cells enter meiosis in rats embryo, and STRA8 and SCP3 could serve as molecular markers for the meiosis in rats. The localization of STRA8 in the nucleus increased the possibility that STRA8 might act as transcription factor or activate transcription to function after birth.

中文翻译：

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减数分裂相关蛋白在不同时期雌性大鼠性腺中的表达及定位


众所周知，雌性哺乳动物卵子发生的关键过程是有丝分裂卵原细胞进入减数分裂。早期对模型动物小鼠的研究表明，视黄酸（RA）反应信号蛋白STRA8（由视黄酸基因8刺激）和减数分裂特异性染色体行为标记蛋白SCP3（联会复合蛋白3）是减数分裂过程中两个关键的分子标记。通过免疫组化、qRT-PCR和Western Blot研究STRA8和SCP3在大鼠卵巢中不同时期的表达。免疫组化结果显示STRA8和SCP3主要在胚胎期表达。并且出生后STRA8在卵巢的细胞质和细胞核中表达。 qRT-PCR和Western Blot结果表明，STRA8和SCP3的mRNA和蛋白水平在胚胎期均有表达。 STRA8和SCP3的表达表明大鼠胚胎生殖细胞进入减数分裂，STRA8和SCP3可以作为大鼠减数分裂的分子标记。 STRA8在细胞核中的定位增加了STRA8作为转录因子或在出生后激活转录发挥功能的可能性。