Herein, thermophilic lipase QLM from Alcaligenes sp. has been successfully immobilized in bio-based metal-organic frameworks (MOFs) through biomimetic mineralization, using zinc acetate and adenine as metal ion and organic ligand, respectively. The morphology and structure of lipase@Bio-MOF was systematically characterized by scanning electron microcopy (SEM), transmission electron microcopy (TEM), powder X-ray diffraction (PXRD) and Fourier transform infrared spectra (FT-IR). The enzyme loading in immobilized enzyme was measured to be 15.9 % by thermogravimetric analysis (TGA). Further, it was demonstrated to possess favorable catalytic activity and stability under high temperature and alkaline conditions and in the presence of metal ions, using the hydrolysis of p-nitrophenyl caprylate as a model. Finally, the immobilized enzyme was successfully applied in the preparation of biodiesel through the trans-esterification of sunflower oil with methanol, obtaining a conversion of >60 % at a high oil/methanol ratio of 8:1. Meanwhile, it showed excellent recyclability during the biodiesel production, and no changes of morphology and crystal structure were observed after being used for 3 cycles. Overall, the immobilized lipase in bio-based MOFs provided an economically and environmentally viable biocatalyst for the synthesis of biodiesel.

中文翻译：

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通过仿生矿化构建的生物基金属有机框架中的固定化脂肪酶：用于生物柴油合成的可持续生物催化剂。

在此，来自产碱杆菌属的嗜热脂肪酶 QLM。通过仿生矿化，分别使用乙酸锌和腺嘌呤作为金属离子和有机配体，已经成功地固定在生物基金属有机框架（MOF）中。通过扫描电子显微镜（SEM）、透射电子显微镜（TEM）、粉末X射线衍射（PXRD）和傅里叶变换红外光谱（FT-IR）系统地表征了脂肪酶@Bio-MOF的形貌和结构。通过热重分析 (TGA) 测得固定化酶中的酶载量为 15.9%。此外，以对硝基苯辛酸酯的水解为模型，证明它在高温和碱性条件下以及在金属离子存在下具有良好的催化活性和稳定性。最后，该固定化酶成功应用于葵花油与甲醇的酯交换反应制备生物柴油，在8:1的高油/甲醇比下转化率>60%。同时，它在生物柴油生产过程中表现出良好的可回收性，使用3次循环后形态和晶体结构均未发生变化。总的来说，生物基 MOF 中的固定化脂肪酶为合成生物柴油提供了一种经济和环境可行的生物催化剂。使用3次循环后，形貌和晶体结构均无变化。总的来说，生物基 MOF 中的固定化脂肪酶为合成生物柴油提供了一种经济和环境可行的生物催化剂。使用3次循环后，形貌和晶体结构均无变化。总的来说，生物基 MOF 中的固定化脂肪酶为合成生物柴油提供了一种经济和环境可行的生物催化剂。