Cationic conjugated polymers for enhancing beneficial bacteria adhesion and biofilm formation in gut microbiota.,Colloids and Surfaces B: Biointerfaces

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Cationic conjugated polymers for enhancing beneficial bacteria adhesion and biofilm formation in gut microbiota.
Colloids and Surfaces B: Biointerfaces ( IF 5.4 ) Pub Date : 2020-01-20 , DOI: 10.1016/j.colsurfb.2020.110815
Pengbo Zhang ₁ , Chunling Xu ₂ , Xin Zhou ₃ , Ruilian Qi ₃ , Libing Liu ₃ , Fengting Lv ₃ , Zhengping Li ₄ , Shu Wang ₃

Affiliation

It is important to develop efficient therapeutic methods to maintain a healthy balance among gut microbiota by increasing the beneficial bacteria and decreasing the harmful bacteria. In this work, a cationic polythiophene derivative poly(3-(3'-N,N,N-triethylamino-1'-propyloxy)-4-methyl-2,5-thiophene hydrochloride) (PMNT) with quaternary ammonium groups as side chains has been used for efficiently promoting the initial adhesion and biofilm formation of beneficial bacteria in gut microbiota. Upon addition of PMNT, three species of gut microbiota have an increased biofilm formation ability (216.5 % for Escherichia coli (E. coli), 130.7 % for Bifidobacterium infantis (B. infants) and 47.6 % for Enterococcus faecalis (E. faecalis)). As the initial adhesion of bacteria to a surface is an essential step during biofilm formation, PMNT can promote the attachment of bacteria by forming bacteria /PMNT aggregates which possess more cell-to-cell interactions. RNA sequencing results of bacteria within biofilm indicate that the utilization of carbohydrate and glycan is accelerated in the presence of PMNT, leading to enhanced quorum sensing and biofilm formation of E. coli. After forming biofilm, beneficial bacteria have an enhanced resistance to adverse environmental conditions which is significant for maintaining the balance of gut microbiota. Conjugated polymers exhibit a good potential application in modulating the balance of gut microbiota and development of new probiotics drugs.

中文翻译：

阳离子共轭聚合物，用于增强肠道菌群中有益细菌的附着力和生物膜的形成。

重要的是开发有效的治疗方法，以通过增加有益细菌和减少有害细菌来维持肠道菌群之间的健康平衡。在这项工作中，以季铵盐为侧的阳离子聚噻吩衍生物聚（3-（3'-N，N，N-三乙氨基-1'-丙氧基）-4-甲基-2,5-噻吩盐酸盐）（PMNT）链已被用于有效地促进肠道菌群中有益细菌的初始粘附和生物膜形成。添加PMNT后，三种肠道菌群的生物膜形成能力增强（大肠杆菌（E. coli）为216.5％，婴儿双歧杆菌（B. baby）为130.7％，粪肠球菌（E. faecalis）为47.6％）。。由于细菌最初附着在表面上是生物膜形成过程中必不可少的步骤，PMNT可以通过形成具有更多细胞间相互作用的细菌/ PMNT聚集体来促进细菌的附着。生物膜内细菌的RNA测序结果表明，在PMNT的存在下，碳水化合物和聚糖的利用得以加速，从而导致了增强的群体感应和大肠杆菌生物膜的形成。形成生物膜后，有益细菌对不利的环境条件具有增强的抵抗力，这对于维持肠道菌群的平衡非常重要。共轭聚合物在调节肠道菌群平衡和开发新型益生菌药物方面显示出良好的潜在应用。生物膜内细菌的RNA测序结果表明，在PMNT的存在下，碳水化合物和聚糖的利用得以加速，从而导致了增强的群体感应和大肠杆菌生物膜的形成。形成生物膜后，有益细菌对不利的环境条件具有增强的抵抗力，这对于维持肠道菌群的平衡非常重要。共轭聚合物在调节肠道菌群平衡和开发新型益生菌药物方面显示出良好的潜在应用。生物膜内细菌的RNA测序结果表明，在PMNT的存在下，碳水化合物和聚糖的利用得以加速，从而导致了增强的群体感应和大肠杆菌生物膜的形成。形成生物膜后，有益细菌对不利的环境条件具有增强的抵抗力，这对于维持肠道菌群的平衡非常重要。共轭聚合物在调节肠道菌群平衡和开发新型益生菌药物方面显示出良好的潜在应用。

更新日期：2020-01-21

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