As an organic waste of dairy manufacture, cheese whey was considered to be an environmental pollutant because of its high lactose content. Reutilization of lactose from cheese whey for power generation could be a novel solution for dairy industry. In this study, lactose was demonstrated as a fuel for power generation by enzymatic fuel cell (EFC) using cellobiose dehydrogenase (CDH). The enzyme was immobilized and characterized on the mediator modified electrode. To enhance the performance, various factors of this EFC system such as enzyme concentration, reaction pH, and initial concentration of lactose were investigated. The maximum current and power density were obtained at CDH concentration of 47.07 mg/ml and 100 mM lactose at pH 4.5 for EFC. The open circuit voltage and maximum power density of the EFC at optimum conditions were 0.52 V and 2,973 μW/cm², respectively. Additionally, the cheese whey from dairy industry was directly demonstrated in this EFC and power density of 1,839 μW/cm² was obtained. These results indicated positive prospects of cheese whey for the application of power generation, which demonstrated the feasibility of the organic waste from diary manufacture to produce a clean product.

作为乳制品生产的有机废物，奶酪乳清由于其高乳糖含量而被认为是环境污染物。奶酪乳清中乳糖的再利用可用于发电，这可能是奶业的一种新颖解决方案。在这项研究中，乳糖被证明是使用纤维二糖脱氢酶（CDH）通过酶促燃料电池（EFC）发电的燃料。将该酶固定在介体修饰电极上并进行表征。为了提高性能，研究了该EFC系统的各种因素，例如酶浓度，反应pH和乳糖的初始浓度。对于EFC，在CDH浓度为47.07 mg / ml和100 mM乳糖（pH为4.5）下获得最大电流和功率密度。在最佳条件下，EFC的开路电压和最大功率密度为0.52 V和2，^2个。另外，在该EFC中直接展示了来自乳业的干酪乳清，并且获得了1,839μW/ cm ^2的功率密度。这些结果表明干酪乳清在发电方面的应用前景良好，证明了乳制品生产中的有机废物生产清洁产品的可行性。