Due to their high surface‐to‐volume ratio, electro‐catalytic activity, biocompatibility and novel electron transport properties, quantum dot (QD) are highly attractive materials for ultra‐sensitive detection of biological macromolecules via bio‐electronic devices. In this study a QD‐based genosensor was developed in which Ga 2 Te 3 ‐based QD was synthesised using a novel aqueous solution approach by mixing 3‐mercaptosuccinic acid (3MSA)‐capped gallium metal precursor with reduced tellurium metal. The results gave original understandings relating to the active material involved in the probe DNA sensing mechanism. The morphological and structural characterisation of the QD was investigated prior to their utilization in DNA sensor construction. High resolution TEM (HR‐TEM) and atomic force microscopy (AFM) images confirmed the spherical and crystalline nature of the QD while X‐ray Photoelectron Spectroscopy (XPS) and X‐ray diffraction (XRD) analysis were able to confirm the oxidation states and formation of the prepared QD. UV‐VIS was capable to find the optical band gap energy and the photostability of the QD. The resultant Ga 2 Te 3 QD together with metal ions confirmed their use for DNA signal detection through their DNA binding mechanism in the genosensor construction. Genosensing in Cs + and Li + ions exhibited higher sensitivity (2.74 ‐ 3.69 μA ng ‐1 mL) and very low detection limits (0.4 pg mL ‐1 ) with the linear dynamic range of 0.1 ‐ 1 ng mL ‐1 .

中文翻译：

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量子点表面对电化学DNA传感机理的影响

由于其高的表面体积比，电催化活性，生物相容性和新颖的电子传输特性，量子点（QD）是通过生物电子设备超灵敏地检测生物大分子的极具吸引力的材料。在这项研究中，开发了一种基于QD的基因传感器，其中通过将3-巯基琥珀酸（3MSA）封端的镓金属前体与还原碲金属混合，使用一种新颖的水溶液方法合成了基于Ga 2 Te 3的QD。结果给出了与探针DNA传感机制中涉及的活性物质有关的原始理解。在将QD用于DNA传感器构建之前，对其进行了形态和结构表征研究。高分辨率TEM（HR‐TEM）和原子力显微镜（AFM）图像证实了QD的球形和晶体性质，而X射线光电子能谱（XPS）和X射线衍射（XRD）分析能够确定氧化态并准备QD。UV-VIS能够找到光学带隙能量和量子点的光稳定性。所得的Ga 2 Te 3 QD与金属离子一起通过基因传感器构造中的DNA结合机制证实了其在DNA信号检测中的用途。Cs +和Li +离子的基因检测显示出更高的灵敏度（2.74-3.69μAng -1 mL）和非常低的检测限（0.4 pg mL -1），线性动态范围为0.1 -1 ng mL -1