The golden mussel proteome and its response to niclosamide: Uncovering rational targets for control or elimination.,Journal of Proteomics

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The golden mussel proteome and its response to niclosamide: Uncovering rational targets for control or elimination.
Journal of Proteomics ( IF 2.8 ) Pub Date : 2020-01-20 , DOI: 10.1016/j.jprot.2020.103651
Ananda Lima Sanson ₁ , Miguel Cosenza-Contreras ₂ , Ricardo DeMarco ₃ , Leandro Xavier Neves ₁ , Bruno Mattei ₄ , Gustavo Gonçalves Silva ₂ , Paulo Henrique Vieira Magalhães ₅ , Milton Hércules Guerra de Andrade ₆ , William Castro-Borges ₆

Affiliation

The Asian invasive species Limnoperna fortunei (Dunker, 1857), known as the golden mussel, causes great economic and environmental damage due to its fixative capacity and accelerated proliferation. Molecular studies for the control of larval and adult forms are of great economic, scientific and technological interest. Here, we first report on the compositional analysis of the L. fortunei proteome obtained through shotgun analysis using LC-MS/MS. Among those 2790 proteins identified, many of them related to secretory processes and membrane receptors. Our second approach consisted in exposing the mollusc to the molluscicide niclosamide to evaluate the induced proteomic alterations. Exposure to niclosamide at 0.25 mg/L for 24 h resulted in a pronounced differential abundance of proteins when compared to those obtained when exposure was reduced to 4 h at 2.3 mg/L. In total, 342 proteins were found differentially expressed in the responsive individuals as revealed by label-free quantitative proteomics. Regarding the affected cell processes were: cell division and differentiation, cytoskeletal organization and compartment acidification (upregulated), and energy metabolism (downregulated). Our findings constitute the first inventory of the expressed proteome of the golden mussel and have the potential to contribute with a more rational proposition of molecular targets for control and monitoring of this species. SIGNIFICANCE: With the recent availability of transcriptomic and genomic data applied to L. fortunei the timing is right to interrogate its putative gene repertoire using proteomic techniques. These have the potential to validate the existence of the predicted genes, infer their relative abundance and quantify their levels as a response to environmental stressors or various agents. Here we provided an inventory of the golden mussel proteome and evaluated its response to the molluscicide niclosamide. The obtained results open new avenues for intervention aimed at its control or elimination, particularly by targeting the various cellular processes that were uncovered.

中文翻译：

贻贝黄金蛋白组及其对烟酰胺的反应：发现控制或消除的合理目标。

亚洲入侵物种Limnoperna fortunei（Dunker，1857年），被称为金贻贝，由于其固定能力和加速扩散而对经济和环境造成巨大破坏。控制幼虫和成虫形式的分子研究具有重大的经济，科学和技术意义。在这里，我们首先报告通过使用LC-MS / MS的gun弹枪分析获得的福氏乳杆菌蛋白质组的组成分析。在鉴定出的2790种蛋白质中，许多与分泌过程和膜受体有关。我们的第二种方法包括将软体动物暴露于软体动物杀灭剂尼克洛沙胺，以评估诱导的蛋白质组学改变。在0暴露于niclosamide。与在2.3 mg / L下将暴露量减少至4 h时所获得的蛋白质相比，在25 mg / L的情况下24小时可产生明显的蛋白质丰度差异。如无标记的定量蛋白质组学所揭示，总共发现342个蛋白质在反应个体中差异表达。关于受影响的细胞过程是：细胞分裂和分化，细胞骨架组织和区室酸化（上调）以及能量代谢（下调）。我们的发现构成了表达贻贝的蛋白质组的第一个清单，并有可能为控制和监测该物种的分子靶标提出更合理的建议。意义：随着转录组学和基因组数据应用于L的最新可用性。正确的时机是使用蛋白质组学技术探究其推定的基因库的时机。这些具有验证所预测基因的存在，推断其相对丰度并量化其水平作为对环境压力源或各种作用因子的响应的潜力。在这里，我们提供了金色贻贝蛋白质组的清单，并评估了其对杀软体动物尼克洛酰胺的反应。获得的结果为以其控制或消除为目标的干预开辟了新途径，尤其是通过针对尚未发现的各种细胞过程进行干预。在这里，我们提供了金色贻贝蛋白质组的清单，并评估了其对杀软体动物尼克洛酰胺的反应。获得的结果为以其控制或消除为目标的干预开辟了新途径，尤其是通过针对尚未发现的各种细胞过程进行干预。在这里，我们提供了金色贻贝蛋白质组的清单，并评估了其对杀软体动物尼克洛酰胺的反应。获得的结果为以其控制或消除为目标的干预开辟了新途径，尤其是通过针对尚未发现的各种细胞过程进行干预。

更新日期：2020-01-21

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