Liquid biopsy has emerged in the last ten years as an appealing noninvasive strategy to support early cancer diagnosis and follow-up interventions. However, conventional liquid biopsy strategies involving specified biomarkers have encountered unexpected inconsistencies stemming from the use of different analytical methodologies. Recent reports have repeatedly demonstrated that integrated detection of multiple liquid biopsy biomarkers can significantly improve diagnostic performance by eliminating the influence of intratumoral heterogeneity. Herein, we review the progress in the field of liquid biopsy and propose a novel integrated liquid biopsy framework consisting of three categories: elementary, intermediate, and advanced integration. We also summarize the merits of the integration strategy and propose a roadmap toward refining cancer diagnosis, metastasis surveillance, and prognostication.

在过去的十年中，液体活检已成为一种吸引人的非侵入性策略，可支持早期癌症诊断和后续干预。然而，涉及特定生物标志物的常规液体活检策略由于使用不同的分析方法而遇到了意想不到的矛盾。最近的报道反复证明，通过消除肿瘤内异质性的影响，多种液体活检生物标志物的整合检测可以显着提高诊断性能。本文中，我们回顾了液体活检领域的进展，并提出了一种新颖的综合性液体活检框架，该框架由三类组成：基础，中级和高级整合。我们还总结了整合策略的优点，并提出了完善癌症诊断的路线图，