The use of non-potable water (such as seawater) is an attractive alternative for water intensive processes such as biomass pretreatment and saccharification steps in the production of biochemicals and biofuels. Identification and application of halotolerant enzymes compatible with high-salt conditions may reduce the energy needed for non-potable water treatment and decrease waste treatment costs. Here we present the biochemical properties of a halotolerant endo-1,4-β-xylanase produced by Aspergillus clavatus in submerged fermentation, using paper sludge (XPS) and sugarcane bagasse (XSCB), and its potential application in the hydrolysis of agroindustrial residues. The peptide mass fingerprint and amino acid sequencing of the XPS and XSCB enzymes showed primary structure similarities with an endo-1,4-β-xylanase from Aspergillus clavatus (XYNA_ASPCL). Both enzyme preparations presented good thermal stability at 50 °C and were stable over a wide range of pH and V_max up to 2450 U/mg for XPS. XPS and XSCB were almost fully stable even after 24 h of incubation in the presence of up to 3 M NaCl, and their activity were not affected by 500 mM NaCl. Both enzyme preparations were capable of hydrolyzing paper sludge and sugarcane bagasse to release reducing sugars. These characteristics make this xylanase attractive to be used in the hydrolysis of biomass, particularly with brackish water or seawater.

非饮用水（例如海水）的使用是水密集型过程（例如生物化学和生物燃料生产中的生物质预处理和糖化步骤）的一种有吸引力的替代方法。鉴定和应用与高盐条件兼容的卤代脱氧核糖核酸酶可以减少非饮用水处理所需的能源，并降低废物处理成本。在这里，我们介绍了由曲霉曲霉产生的卤代内酯-1,4-β-木聚糖酶的生化特性。纸污泥（XPS）和甘蔗渣（XSCB）在深层发酵中的应用，及其在水解农业工业残留物中的潜在应用。XPS和XSCB酶的肽质量指纹图谱和氨基酸序列分析表明，它们与来自曲霉菌（Aspergillus clavatus）的内切1,4-β-木聚糖酶（XYNA_ASPCL）的一级结构相似。两种酶制剂均在50°C时表现出良好的热稳定性，并且在宽范围的pH和V _max下均稳定XPS高达2450 U / mg。即使在最多3 M NaCl存在下孵育24小时后，XPS和XSCB也几乎完全稳定，并且其活性不受500 mM NaCl的影响。两种酶制剂都能够水解纸污泥和甘蔗渣以释放还原糖。这些特性使这种木聚糖酶具有吸引力，可用于生物质的水解，特别是在微咸水或海水中。