The study evaluates the protective effect of mangiferin on osteosarcoma cell proliferation and metastasis. Saos-2 and U2OS cells were treated with mangiferin (25, 50, 75 and 100 µM) for 72 h. Mangiferin reduced the cell viability, invasion, and cell adhesion and migration rate. Matrix metalloproteinases-2/9 (MMP-2/9) mRNA expression was reduced significantly, while the levels of tissue inhibitors of metalloproteinases-1/2 (TIMP-1/2) were elevated in Saos-2 and U2OS cells. Mangiferin treatment significantly reduced parathyroid hormone receptor 1 (PTHR1) mRNA and protein expression by more than 0.5-fold in both osteosarcoma cells. In addition, the immunofluorescent analysis also showed decreased PTHR1 expression following treatment with mangiferin. In summary, we have demonstrated that treatment with mangiferin reduces cell viability, proliferation, invasion, adhesion and migration, and induces apoptosis of osteosarcoma cells. Therefore, treatment with mangiferin can be effective agent in inhibiting growth and inducing apoptosis in osteosarcoma cells. Our experimental results provide evidence for the therapeutic effect of mangiferin in osteosarcoma cells.

中文翻译：

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芒果苷通过下调金属蛋白酶-1/2和甲状旁腺激素受体1的表达来抑制人类转移性骨肉瘤细胞的生长。

该研究评估了芒果苷对骨肉瘤细胞增殖和转移的保护作用。Saos-2和U2OS细胞用芒果苷（25、50、75和100 µM）处理72小时。芒果苷降低了细胞活力，侵袭以及细胞粘附和迁移速率。在Saos-2和U2OS细胞中，基质金属蛋白酶-2/9（MMP-2 / 9）mRNA表达显着降低，而金属蛋白酶-1/2（TIMP-1 / 2）的组织抑制剂水平升高。芒果苷治疗在两个骨肉瘤细胞中均显着降低了甲状旁腺激素受体1（PTHR1）mRNA和蛋白质表达，超过0.5倍。另外，免疫荧光分析还显示，用芒果苷处理后PTHR1表达降低。总而言之，我们证明了用芒果苷治疗会降低细胞活力，增殖，侵袭，粘附和迁移，并诱导骨肉瘤细胞凋亡。因此，用芒果苷治疗可以有效地抑制骨肉瘤细胞的生长并诱导其凋亡。我们的实验结果为芒果苷对骨肉瘤细胞的治疗作用提供了证据。