Abstract

A novel liquid–liquid extraction method of phenolic compounds from extra virgin olive using microfluidic technique was developed. The microdevice of polydimethylsiloxane (PDMS) was designed in two parts; the first one to carry out the extraction of polyphenols using only an alkaline aqueous solution and the second one to develop the reaction product using Folin–Ciocalteu reagent, which was measured at 730 nm at the microchip exit. Hydrodynamic and chemical parameters, such as flowrates of extraction and reaction, length of microchannels, extraction pH, extraction buffer concentration, and concentration of Folin–Ciocalteu reagent, were evaluated. Although all parameters were important, the results showed that pH, carbonate buffer concentration, and the Folin–Ciocalteu reagent concentration were significant factors and that the increase in the length of the extraction coil enhanced the extraction percentage. The results showed higher extraction efficiency by the microfluidic method, between 46 and 67%, than for the other two batch extraction methods.

中文翻译：

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实验室中从特级初榨橄榄油中酚类化合物的芯片提取

摘要

开发了一种利用微流技术从特级初榨橄榄油中液-液萃取酚类化合物的新方法。聚二甲基硅氧烷（PDMS）的微型器件分为两部分设计：第一个仅使用碱性水溶液进行多酚的提取，第二个使用Folin–Ciocalteu试剂开发反应产物，该试剂在微芯片出口处于730 nm处测量。评估了流体动力学和化学参数，例如萃取和反应的流量，微通道的长度，萃取pH，萃取缓冲液浓度以及Folin–Ciocalteu试剂的浓度。尽管所有参数都很重要，但结果表明pH，碳酸盐缓冲液浓度，以及Folin–Ciocalteu试剂浓度是重要因素，并且提取盘管长度的增加会提高提取率。结果表明，与其他两种分批提取方法相比，微流体方法的提取效率更高，介于46％和67％之间。