The lipidome of royal jelly (RJ) consists of medium-chained (8-12 carbon atoms) free fatty acids. We present herein a liquid chromatography-high resolution mass spectrometry (HRMS) method that permits the determination of RJ fatty acids and at the same time the detection of suspect fatty acids. The method allows for the direct quantification of seven free fatty acids of RJ, avoiding any derivatization step. It was validated and applied in seven RJ samples, where the major RJ fatty acid trans-10-hydroxy-2-decenoic acid (10-HDA) was found to vary from 0.771 ± 0.08 to 0.928 ± 0.04 g/100 g fresh RJ. Four additional suspect fatty acids were simultaneously detected taking advantage of the HRMS detection.

中文翻译：

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通过液相色谱-高分辨率质谱 (LC-HRMS) 研究蜂王浆游离脂肪酸。


蜂王浆 (RJ) 的脂质组由中链（8-12 个碳原子）游离脂肪酸组成。我们在此提出了一种液相色谱-高分辨率质谱 (HRMS) 方法，该方法允许测定 RJ 脂肪酸，同时检测可疑脂肪酸。该方法可以直接定量 RJ 的七种游离脂肪酸，避免任何衍生化步骤。它经过验证并应用于七个 RJ 样品，其中主要 RJ 脂肪酸反式 10-羟基-2-癸烯酸 (10-HDA) 变化范围为 0.771 ± 0.08 至 0.928 ± 0.04 g/100 g 新鲜 RJ。利用 HRMS 检测同时检测到另外四种可疑脂肪酸。