In this work, biocompatibility of bacterial cellulose (BC) was assessed as the scaffold for corneal stroma replacement. Biocompatibility was evaluated by examining rabbit corneal epithelial and stromal cells cultured on the BC scaffold. The growth of primary cells was assessed by optical microscope, scanning electron microscope (SEM), and transmission electron microscope (TEM). Live/dead viability/cytotoxicity assay and CCK-8 assay were used to evaluate cell survival. BC was surgically implanted in vivo into a stromal pocket. During a 3-month follow-up, the biocompatibility of BC was assessed. We found that epithelial and stromal cells grew well on BC and showed a survival rate of nearly 100%. The SEM examination for both kinds of cell showed abundant leafy protrusions, spherical projections, filopodia, cytoskeletons, and cellular interconnections. The stromal cells cultured on BC arranged regularly. TEM observation revealed normal cellular microstructure and a tight adhesion to the BC membrane. In vivo observation confirmed the optical transparency of BC during 3-month follow-up. The results demonstrated that BC had good biocompatibility for the tissue engineering of corneal stroma.

中文翻译：

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细菌纤维素作为组织工程角膜基质支架材料的生物相容性评估

在这项工作中，细菌纤维素（BC）的生物相容性被评估为角膜基质置换的支架。通过检查在BC支架上培养的兔角膜上皮和基质细胞来评估生物相容性。通过光学显微镜，扫描电子显微镜（SEM）和透射电子显微镜（TEM）评估原代细胞的生长。活/死活力/细胞毒性测定和CCK-8测定用于评估细胞存活。BC通过外科手术体内植入基质袋中。在3个月的随访中，评估了BC的生物相容性。我们发现上皮细胞和基质细胞在BC上生长良好，并显示出近100％的存活率。两种细胞的SEM检查均显示大量叶状突起，球形突起，丝状伪足，细胞骨架和细胞相互连接。在BC上培养的基质细胞排列规则。TEM观察显示正常的细胞微结构和对BC膜的紧密粘附。体内观察证实了3个月的随访期间BC的光学透明性。结果表明，BC对角膜基质组织工程具有良好的生物相容性。