Novel laccases have promising and valuable applications in biorefineries. This investigation documents, for the first time, the potential of depolymerising and repolymerising lignin by the secretome, rich in laccases, from a newly isolated white-rot basidiomycete Marasmiellus palmivorus VE111, for further saccharification and ethanolic fermentation steps. Proteomic analyses of the secretome of M. palmivorus show that laccases are the most predominant enzyme released by this fungus. The whole crude enzymatic broth is used for the delignification of lignin in Eucalyptus globulus wood, with the aim of enhancing the saccharification by cellulolytic and xylanolytic enzymes from Penicillium echinulatum S1M29. In addition, two different strategies, namely, laccase treatment before and after enzymatic hydrolysis, are employed to detoxify steam-exploded E. globulus wood. The objective is to increase the fermentative performance by removing substances formed during the feedstock pretreatment that can inhibit microbial fermentation. The E. globulus wood delignification results in a 31% decrease in the lignin content and a 10% increase in the glucose yield after hydrolysis. An important finding of the present work is the successful wood delignification in the absence of laccase mediators. This laccase-rich preparation also demonstrates its potential in removing the phenolic inhibitors present in steam-exploded E. globulus wood, increasing the ethanol yield by an additional 10%. Furthermore, it is important to highlight that these findings are achieved in the absence of commercial enzymes, making M. palmivorus laccases a potential candidate not only for the production of biofuels but also for the generation of lignin-derived aromatic compounds for different applications in the biotechnology industry.

新型漆酶在生物精炼厂具有广阔的应用前景。这项研究首次记录了从新分离的白腐担子菌Marasmiellus palmivorus VE111中富含漆酶的分泌酶使木质素解聚和再聚合的潜力，可用于进一步的糖化和乙醇发酵步骤。棕榈果分枝杆菌分泌蛋白的蛋白质组学分析表明，漆酶是该真菌释放的最主要的酶。整个粗酶液被用于在木质素脱木素蓝桉木，与由从纤维素和木聚糖分解酶增强糖化的目的青霉echinulatumS1M29。另外，采用两种不同的策略，即酶促水解之前和之后的漆酶处理，对蒸汽爆炸的大肠埃希氏球菌进行解毒。目的是通过去除在原料预处理期间形成的可抑制微生物发酵的物质来提高发酵性能。的蓝桉木脱木素导致在木质素含量31％的下降和在水解后葡萄糖产量增加了10％。当前工作的重要发现是在没有漆酶介体的情况下成功的木材脱木素。这种富含漆酶的制剂还证明了其去除蒸汽爆炸的大肠埃希氏球菌中存在的酚类抑制剂的潜力。木材，使乙醇的收率增加了10％。此外，重要的是要强调指出，这些发现是在不存在商业酶的情况下获得的，这使得棕榈分枝杆菌漆酶不仅可能用于生产生物燃料，而且可以用于生成木质素衍生的芳香族化合物，以用于不同用途。生物技术产业。