Xylo- and arabinoxylo-oligosaccharides (XOS and AXOS) are of interest for their prebiotic activity. The production of these oligomers might be accompanied with monosaccharides. The measurement of both oligosaccharides and monosaccharides usually requires two methods. The current work presents an HPAEC-PAD method based on gradient elution of aqueous solvents sodium hydroxide and sodium acetate, in contrast to conventional isocratic elution, for the simultaneous separation of 16 standards of monosaccharides, xylo-oligosaccharides, arabinoxylo-oligosaccharides and uronic acids using CarboPac PA 200 column. The presented method showed a stable baseline and high-resolution separation of the standards. The method showed acceptable accuracy and precision. Limits of Detection and Quantitation (LOD and LOQ) were estimated for all the standards. The method was applied to measure the activity of a commercial endoxylanase on wheat bran; a steady release of xylose monosaccharide was observed. Enzyme action on oligosaccharide standards showed a preference for the larger oligosaccharides.

中文翻译：

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使用HPAEC-PAD同时测定谷物单糖，木糖和阿拉伯糖基寡糖以及糖醛酸。

木糖和阿拉伯糖寡糖（XOS和AXOS）因其益生元活性而受到关注。这些低聚物的产生可能伴随有单糖。寡糖和单糖的测量通常需要两种方法。当前的工作提出了一种HPAEC-PAD方法，与传统的等度洗脱相比，该方法基于水性溶剂氢氧化钠和乙酸钠的梯度洗脱，可同时分离16种标准的单糖，木糖，寡糖，阿拉伯糖基低聚糖和糖醛酸CarboPac PA 200色谱柱。提出的方法显示了稳定的基线和标准品的高分辨率分离。该方法显示出可接受的准确性和精确度。估计所有标准的检测和定量限（LOD和LOQ）。该方法用于测定市售内切木聚糖酶对麦麸的活性。观察到木糖单糖的稳定释放。酶对低聚糖标准物的作用显示出对较大的低聚糖的偏爱。