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Biocontrol of tobacco black shank disease (Phytophthora nicotianae) by Bacillus velezensis Ba168
Pesticide Biochemistry and Physiology ( IF 4.2 ) Pub Date : 2020-05-01 , DOI: 10.1016/j.pestbp.2020.01.004 Dongsheng Guo 1 , Chenhong Yuan 1 , Yunyan Luo 1 , YaHan Chen 1 , Meihuan Lu 1 , Guochan Chen 2 , Guangwei Ren 3 , Chuanbin Cui 4 , Jiatao Zhang 4 , Derong An 1
Pesticide Biochemistry and Physiology ( IF 4.2 ) Pub Date : 2020-05-01 , DOI: 10.1016/j.pestbp.2020.01.004 Dongsheng Guo 1 , Chenhong Yuan 1 , Yunyan Luo 1 , YaHan Chen 1 , Meihuan Lu 1 , Guochan Chen 2 , Guangwei Ren 3 , Chuanbin Cui 4 , Jiatao Zhang 4 , Derong An 1
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Tobacco black shank (TBS) caused by Phytophthora nicotianae is destructive to almost all tobacco cultivars and is widespread in many tobacco-growing countries. Through lab study and field test, we isolated plant growth-promoting rhizobacteria (PGPR) strain Ba168 which is a promising biocontrol strain of TBS. Ba168 was isolated from 168 soil samples and identified as Bacillus velezensis by its genetic and phenotypic characteristics. A susceptibility test indicated that the P. nicotianae antagonistic materials of Ba168 in extracellular metabolites were composed of effective and stable proteins/peptides. P. nicotianae's growth was suppressed by the ammonium sulfate precipitation of Ba168 culture filtrates (ASPBa) at a minimum inhibitory concentration of 5 μg/mL. Extracellular conductivity, pH, and the wet/dry weights of P. nicotianae's mycelia, along with scanning electron microscope analysis, suggested that Ba168-derived proteins/peptides could effectively inhibit P. nicotianae by causing irreversible damage to its cell walls and membranes. Protein identification of ASPBa supported these results and identified many key proteins responsible for various biocontrol-related pathways. Field assays of TBS control efficacy of many PGPRs and agrochemicals showed that all PGPR preparations reduced the disease index of tobacco, but Ba168 was the most effective. These results demonstrated the importance of Bacillus-derived proteins/peptides in the inhibition of P. nicotianae through irreversible damage to its cell wall and membrane; and the effectiveness of PGPR strain B. velezensis Ba168 for biocontrol of the soil-borne disease caused by P. nicotianae.
中文翻译：
贝莱斯芽孢杆菌 Ba168 对烟草黑胫病（Phytophthora nicotianae）的生物防治
由烟草疫霉引起的烟草黑胫病（TBS）对几乎所有烟草品种都有破坏性，并且在许多烟草种植国家广泛传播。通过实验室研究和田间试验，我们分离得到了植物根际促生菌（PGPR）菌株Ba168，它是一种很有前景的TBS生防菌株。 Ba168是从168份土壤样品中分离得到的，通过其遗传和表型特征鉴定为贝莱斯芽孢杆菌（Bacillus velezensis）。药敏试验表明，烟草胞外代谢产物Ba168拮抗物质由有效、稳定的蛋白/肽组成。 Ba168 培养物滤液 (ASPBa) 的硫酸铵沉淀以 5 μg/mL 的最低抑制浓度抑制烟草假单胞菌的生长。烟草假单胞菌菌丝体的细胞外电导率、pH 值和湿/干重以及扫描电子显微镜分析表明，Ba168 衍生的蛋白质/肽可以通过对其细胞壁和细胞膜造成不可逆的损伤来有效抑制烟草假单胞菌。 ASPBa 的蛋白质鉴定支持了这些结果，并鉴定了许多负责各种生物防治相关途径的关键蛋白质。对多种PGPR和农用化学品的TBS防效田间试验表明，所有PGPR制剂均降低了烟草的病害指数，但Ba168最为有效。这些结果证明了芽孢杆菌衍生的蛋白质/肽通过对其细胞壁和细胞膜造成不可逆转的损伤来抑制烟草假单胞菌的重要性；以及 PGPR 菌株 B. velezensis Ba168 对生物防治由 P. nicotianae 引起的土传病害的有效性。
更新日期：2020-05-01
中文翻译：
贝莱斯芽孢杆菌 Ba168 对烟草黑胫病（Phytophthora nicotianae）的生物防治
由烟草疫霉引起的烟草黑胫病（TBS）对几乎所有烟草品种都有破坏性，并且在许多烟草种植国家广泛传播。通过实验室研究和田间试验，我们分离得到了植物根际促生菌（PGPR）菌株Ba168，它是一种很有前景的TBS生防菌株。 Ba168是从168份土壤样品中分离得到的，通过其遗传和表型特征鉴定为贝莱斯芽孢杆菌（Bacillus velezensis）。药敏试验表明，烟草胞外代谢产物Ba168拮抗物质由有效、稳定的蛋白/肽组成。 Ba168 培养物滤液 (ASPBa) 的硫酸铵沉淀以 5 μg/mL 的最低抑制浓度抑制烟草假单胞菌的生长。烟草假单胞菌菌丝体的细胞外电导率、pH 值和湿/干重以及扫描电子显微镜分析表明，Ba168 衍生的蛋白质/肽可以通过对其细胞壁和细胞膜造成不可逆的损伤来有效抑制烟草假单胞菌。 ASPBa 的蛋白质鉴定支持了这些结果，并鉴定了许多负责各种生物防治相关途径的关键蛋白质。对多种PGPR和农用化学品的TBS防效田间试验表明，所有PGPR制剂均降低了烟草的病害指数，但Ba168最为有效。这些结果证明了芽孢杆菌衍生的蛋白质/肽通过对其细胞壁和细胞膜造成不可逆转的损伤来抑制烟草假单胞菌的重要性；以及 PGPR 菌株 B. velezensis Ba168 对生物防治由 P. nicotianae 引起的土传病害的有效性。
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