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Molecularly imprinted microparticles (microMIPs) embedded with reduced graphene oxide for capture and destruction of E. coli in drinking water
Biomaterials Advances ( IF 5.5 ) Pub Date : 2020-01-16 , DOI: 10.1016/j.msec.2020.110672
Juili S. Mankar , Madhu D. Sharma , Sadhana S. Rayalu , Reddithota J. Krupadam

In this article for the first time, we have reported, a facile way for the creation of E. coli impressions in the polymer for selective capture and to destroy E. coli in drinking water. This microporous imprinted polymer has shown the existence of micrometer size rod shape cavities with the population of 2.45 × 102 ± 60 imprints per cm2. Adsorption capacity of the polymer for E. coli was 103 CFU mg1. This microporous imprinted polymer captured 99% of the bacteria within 30 min at initial concentration of 109 CFU mL1. The non-imprinted polymer prepared without the bacteria imprinting reported only 40% of the bacteria removal even after 60 min. The reduced graphene oxide was embedded in the microporous imprinted polymer and it reported minimum inhibitory concentration at 7.4 mg L1. Within 10 min, reduced graphene oxide completely kills the E. coli while microporous imprinted polymer was embedded with the reduced graphene oxide takes about 13 min to disinfect the water. The reduced graphene oxide nanoparticles were near the imprinted cavity to generate localized temperature between 180 and 210 °C to kill the bacterial cells trapped inside the imprinted cavities of the polymer. The thermal atomic force microscope with the specialized heated probe tips were used to determine the localized temperature in the polymers. The localized thermal energy would be responsible for the production of superoxides, which were as similar to photolysis reactions, and would be further improving antibacterial activity. The combination of selective capture and destruction of pathogens in a single molecular construct improves disinfection of drinking water.



中文翻译:

嵌入有用于捕获和破坏还原的石墨烯氧化物的分子印迹微粒(的microMIPS)E. 大肠杆菌饮用水中

在这篇文章中,第一次,我们已经报道,为创造一个便利途径E. 大肠杆菌聚合物的选择性捕获,展示次数和破坏大肠杆菌饮用水。这种微孔印迹聚合物已经显示微米尺寸棒状的空腔的存在与2.45×10人口2 每厘米±60印记2。该聚合物用于吸附容量E. 大肠杆菌为10 3  CFU毫克- 1。这种微孔印迹聚合物所捕获的细菌的99%在30分钟内以10的初始浓度9  CFU毫升- 1。制备的没有印迹细菌的非印迹聚合物即使在60分钟后也仅报告了40%的细菌去除率。还原氧化石墨烯嵌入微孔印迹聚合物中,并将其在7.4毫克的L报道最小抑制浓度- 1。在10分钟内,还原的石墨烯氧化物完全杀死E. 大肠杆菌而将微孔印迹聚合物嵌入还原的氧化石墨烯中大约需要13分钟来对水进行消毒。还原的氧化石墨烯纳米颗粒靠近压印腔,以产生180至210°C的局部温度,以杀死困在聚合物压印腔内的细菌细胞。使用带有专用加热探针头的热原子力显微镜来确定聚合物中的局部温度。局部热能将负责产生超氧化物,这与光解反应相似,并且将进一步提高抗菌活性。在单个分子构建物中选择性捕获和破坏病原体的组合可改善饮用水的消毒效果。

更新日期:2020-01-16
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