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Oleic acid protects against cadmium induced cardiac and hepatic tissue injury in male Wistar rats: A mechanistic study.
Life Sciences ( IF 5.2 ) Pub Date : 2020-01-17 , DOI: 10.1016/j.lfs.2020.117324 Bharati Bhattacharjee 1 , Palash Kumar Pal 1 , Aindrila Chattopadhyay 2 , Debasish Bandyopadhyay 1
Life Sciences ( IF 5.2 ) Pub Date : 2020-01-17 , DOI: 10.1016/j.lfs.2020.117324 Bharati Bhattacharjee 1 , Palash Kumar Pal 1 , Aindrila Chattopadhyay 2 , Debasish Bandyopadhyay 1
Affiliation
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AIMS
The aim of the present study was to evaluate the possible antioxidant role of oleic acid (OA) against Cd-induced injuries in the heart and liver tissues of male Wistar rats.
MAIN METHODS
Rats were treated with either vehicle (control), or OA (10 mg/kg b.w., fed orally), or Cd (0.44 mg/kg b.w., s.c.), or both (OA + Cd) for 15 days. Following completion of the treatment period, biomarkers of organ damage and oxidative stress including ROS, activities of antioxidant enzymes and their level, activities of Krebs cycle enzymes and respiratory chain enzymes were measured. Levels of interleukins (IL-1β, IL-6, IL-10), tumor necrosis factor (TNF-α) and nuclear factor kappa B (NFκB) were estimated to evaluate the state of inflammation. In addition, changes in mitochondrial membrane potential and status of cytochrome c (Cyt c) were also studied.
KEY FINDINGS
Pre-treatment of rats with OA significantly protected against Cd-induced detrimental changes possibly by decreasing endogenous ROS through regulation of antioxidant defense system, inflammatory responses and activities of metabolic enzymes. Moreover, OA was also found to restore mitochondrial membrane potential possibly by regulating Cyt c leakage thereby increasing mitochondrial viability.
SIGNIFICANCE
Our results for the first time demonstrated systematically that OA provided protection against Cd-induced oxidative stress mediated injuries in rat heart and liver tissues through its antioxidant mechanism. The results raise the possibility of using OA singly or in combination with other antioxidants or diet in the treatment of situations arising due to oxidative stress and may have future therapeutic relevance.
中文翻译：
油酸可防止镉对雄性Wistar大鼠心脏和肝脏组织的伤害：一项机理研究。
目的本研究的目的是评估油酸（OA）对Cd诱导的雄性Wistar大鼠心脏和肝脏组织损伤的抗氧化作用。主要方法用赋形剂（对照组）或OA（10 mg / kg bw，口服）或Cd（0.44 mg / kg bw，sc）或两者（OA + Cd）治疗大鼠15天。治疗期结束后，测量器官损伤和氧化应激的生物标志物，包括ROS，抗氧化酶的活性及其水平，克雷布斯循环酶和呼吸链酶的活性。估计白介素（IL-1β，IL-6，IL-10），肿瘤坏死因子（TNF-α）和核因子κB（NFκB）的水平以评估炎症状态。此外，还研究了线粒体膜电位的变化和细胞色素c（Cyt c）的状态。主要发现OA大鼠的预处理可以通过调节抗氧化剂防御系统，炎症反应和代谢酶的活性来降低内源性ROS，从而显着保护Cd诱导的有害变化。此外，还发现OA可能通过调节Cyt c泄漏从而恢复线粒体膜电位，从而增加线粒体生存力。意义我们的首次研究结果系统地证明了OA通过其抗氧化机制为保护Cd诱导的大鼠心脏和肝脏组织的氧化应激介导的损伤提供了保护。结果提高了单独使用OA或与其他抗氧化剂或饮食组合使用OA治疗由氧化应激引起的情况的可能性，并且可能具有未来的治疗意义。
更新日期：2020-01-17
中文翻译：
油酸可防止镉对雄性Wistar大鼠心脏和肝脏组织的伤害：一项机理研究。
目的本研究的目的是评估油酸（OA）对Cd诱导的雄性Wistar大鼠心脏和肝脏组织损伤的抗氧化作用。主要方法用赋形剂（对照组）或OA（10 mg / kg bw，口服）或Cd（0.44 mg / kg bw，sc）或两者（OA + Cd）治疗大鼠15天。治疗期结束后，测量器官损伤和氧化应激的生物标志物，包括ROS，抗氧化酶的活性及其水平，克雷布斯循环酶和呼吸链酶的活性。估计白介素（IL-1β，IL-6，IL-10），肿瘤坏死因子（TNF-α）和核因子κB（NFκB）的水平以评估炎症状态。此外，还研究了线粒体膜电位的变化和细胞色素c（Cyt c）的状态。主要发现OA大鼠的预处理可以通过调节抗氧化剂防御系统，炎症反应和代谢酶的活性来降低内源性ROS，从而显着保护Cd诱导的有害变化。此外，还发现OA可能通过调节Cyt c泄漏从而恢复线粒体膜电位，从而增加线粒体生存力。意义我们的首次研究结果系统地证明了OA通过其抗氧化机制为保护Cd诱导的大鼠心脏和肝脏组织的氧化应激介导的损伤提供了保护。结果提高了单独使用OA或与其他抗氧化剂或饮食组合使用OA治疗由氧化应激引起的情况的可能性，并且可能具有未来的治疗意义。
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