Since HOCl plays an indispensable role in a variety of physiological and pathological processes, monitoring the level of hypochlorous acid (HOCl) in living cells is critical. Based on this, it is necessary to develop a convenient and simple technique to detect HOCl in living cells. In this contribution, a simple 2-hydroxy-6-morpholino-1H-benzo[de]isoquinoline-1,3(2 H)-dione (NDS) was developed as fluorescent probe for monitoring HOCl in live cells. Probe NDS exhibited preeminent specificity towards HOCl over other various bioactive substances. In addition, the probe could respond to HOCl in solutions within 16 s and the limit of detection was as low as 105.24 nM. NDS has good lysosomal targeting and has been successfully applied to the expression of exogenous and endogenous lysosomal HOCl in HeLa cells. This study provides a promising tool for studying physiological or pathological related processes.

由于HOCl在各种生理和病理过程中起着不可或缺的作用，因此监测活细胞中次氯酸（HOCl）的水平至关重要。基于此，有必要开发一种方便，简单的技术来检测活细胞中的HOCl。在这种贡献，一个简单的2-羟基-6-吗啉代-1H-苯并[ DE ]异喹啉-1,3（2H） -二酮（NDS）是作为荧光探针用于在活细胞中监测次氯酸。NDS探针对HOCl的特异性优于其他各种生物活性物质。此外，该探针可在16 s内对溶液中的HOCl作出反应，检测限低至105.24 nM。NDS具有良好的溶酶体靶向性，已成功应用于HeLa细胞中外源和内源性溶酶体HOCl的表达。这项研究为研究生理或病理相关过程提供了一个有前途的工具。