Abstract The effect of low-intensity ultrasound irradiation was studied to improve the co-production for pectinase, cellulase, and xylanase enzymes using Bacillus subtilis ABDR01. Different parameters such as ultrasonic irradiation at the different growth phases of the bacterial strain, ultrasound power, irradiation duration, and irradiation duty cycle were assessed. Sonication with 90 W ultrasound power, 25 kHz frequency with 70 % duty cycle for 5 min at 6 h of bacterial growth phase gave the maximum productions of 87.82 U/ mL pectinase 22.17 U/ mL cellulase and 137.95 U/ mL xylanase respectively. The enzyme activity of pectinase, cellulase, and xylanase was enhanced by about 38.15 %, 53.77 %, and 24.59 %, respectively, compared to non-sonicated control cultivation. This optimized low-frequency ultrasound irradiation to bacterial cells enhanced the nutrient uptake rate and increased the cell wall permeability, which results in higher enzyme productivity. Our results signify the effectiveness of low-frequency ultrasound irradiation for improved enzyme yields and hyperactivation during microbial fermentation.

中文翻译：

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超声波照射下枯草芽孢杆菌 ABDR01 中果胶酶、纤维素酶和木聚糖酶的协同生产增强

摘要 利用枯草芽孢杆菌ABDR01，研究了低强度超声辐照对提高果胶酶、纤维素酶和木聚糖酶的协同生产的影响。评估了不同参数，例如细菌菌株不同生长阶段的超声辐照、超声功率、辐照持续时间和辐照占空比。以 90 W 超声功率、25 kHz 频率和 70% 占空比在 6 小时细菌生长期超声处理 5 分钟，分别产生 87.82 U/mL 果胶酶、22.17 U/mL 纤维素酶和 137.95 U/mL 木聚糖酶的最大产量。与非超声处理的对照培养相比，果胶酶、纤维素酶和木聚糖酶的酶活性分别提高了约 38.15%、53.77% 和 24.59%。这种优化的对细菌细胞的低频超声照射提高了营养吸收率并增加了细胞壁通透性，从而提高了酶的生产力。我们的结果表明低频超声辐照在微生物发酵过程中提高酶产量和过度活化的有效性。