Abstract Batch experiments were conducted to study bioelectrochemical enhancement of the anaerobic digestion (AD) of alkaline-pretreated sewage sludge in microbial electrolysis cell (MEC) in the presence or absence of phosphate buffer solution (PBS). Experimental results showed that the maximum methane production rate was increased from 0.18 mL-CH4/(mLreactor·d) (control) to 0.2 mL-CH4/(mLreactor·d) in the presence of PBS and cumulative methane production was 1.4-fold higher than that of control. The initial concentrations of SCOD, protein, polysaccharide and VFAs in the presence of PBS were slightly higher than those of control, suggesting that PBS might facilitate the release of organics into mixed liquor. The microbial community analysis results showed that the microbial community with PBS had higher diversity and Bacteroidetes and Firmicutes were the two most abundant phyla in the communities. Moreover, the PBS addition could enhance the growth of aceticlastic methanogens (Methanosaeta) and inhibit a portion of hydrogenotrophic methanogens (Methanobacterium), possibly due to the different cell wall composition.

中文翻译：

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PBS对碱性预处理污水污泥进料MEC-AD组合系统甲烷产量的影响

摘要 通过批量实验研究了在磷酸盐缓冲溶液 (PBS) 存在或不存在的情况下，微生物电解池 (MEC) 中碱性预处理污水污泥厌氧消化 (AD) 的生物电化学增强作用。实验结果表明，在 PBS 存在下，最大甲烷产量从 0.18 mL-CH4/(mLreactor·d)（对照）增加到 0.2 mL-CH4/(mLreactor·d)，累积甲烷产量提高了 1.4 倍比控制。在 PBS 存在下，SCOD、蛋白质、多糖和 VFAs 的初始浓度略高于对照，表明 PBS 可能促进有机物释放到混合液中。微生物群落分析结果表明，含PBS的微生物群落具有更高的多样性，拟杆菌门和厚壁菌门是群落中最丰富的两个门。此外，加入 PBS 可以促进醋酸产甲烷菌 (Methanosaeta) 的生长并抑制一部分氢营养型产甲烷菌 (Methanobacterium)，这可能是由于细胞壁组成不同。