Extraction of photosynthetic electron for bioelectrical energy harvesting is an efficient approach to utilize solar energy harvested by photosynthetic microorganisms. To explore this approach in natural and artificial photosynthetic system in which alga and photosynthetic bacteria are usually coexisted, the intracellular electron extraction from mixed photosynthetic consortium of Chlorella vulgaris and Rhodopseudomonas palustris was investigated under three-electrode mode by holding working electrode at different potentials. The mixed-culture biofilm grown at 0 V exhibited a maximum Coulomb efficiency of 42.12% while the peak current (12.2 mA) was 8.07, 1.5, 2.97 and 4.65 fold higher than that produced at −0.4 (1.5 mA), −0.2 (8.06 mA), 0.2 (4.08 mA) and 0.4 V (2.6 mA), respectively. The electrode potential can regulate the dominant species within the biofilm. Large enrichment of Rhodopseudomonas palustris in the biofilm was responsible for the high photosynthetic electron extraction efficiency at 0 V since the photosynthetic electrons extracted by the electrode were mainly derived from photoheterotrophic metabolism of Rhodopseudomonas palustris. Extraction of equivalent amounts of intracellular electron from Chlorella vulgaris required higher potential than that from Rhodopseudomonas palustris and was highly dependent on the presence of exogenous electron mediator. As an electron sacrificer, photosynthetic oxygen released by Chlorella vulgaris could complete electron with electrode. Intracellular electrons can also be extracted from dark respiration, but the peak current (6.4 mA) was 47.54% lower than that produced under illumination (12.2 mA) due to low exoelectrogenic activity of biofilm.

用于生物电能收集的光合电子的提取是一种利用光合微生物收集的太阳能的有效方法。为了在藻类和光合细菌通常共存的自然和人工光合作用系统中探索这种方法，从寻常小球藻和大红假单胞菌的光合作用联合体中提取细胞内电子通过将工作电极保持在不同电位下，在三电极模式下进行了研究。在0 V下生长的混合培养生物膜显示出最大库仑效率为42.12％，而峰值电流（12.2 mA）则比-0.4（1.5 mA），-0.2（8.06）时高8.07、1.5、2.97和4.65倍mA），0.2（4.08 mA）和0.4 V（2.6 mA）。电极电位可以调节生物膜内的主要物质。由于电极提取的光合电子主要来自光合养藻，而生物膜中大量富集的光合养藻是造成0 V时高光合电子提取效率的原因。。从寻常小球藻中提取等量的细胞内电子所需的电势要比从大红假单胞菌中提取的高，并且高度依赖于外源电子介体的存在。小球藻释放出的光合作用氧作为电子牺牲剂可以通过电极完成电子。也可以从暗呼吸中提取细胞内电子，但是由于生物膜的外生电活性低，峰值电流（6.4 mA）比在光照下产生的峰值电流（12.2 mA）低47.54％。