Nanomaterials with enzyme-like characteristics (also called nanozymes) have attracted increasing attention in the area of analytical chemistry. Nevertheless, most of the nanozymes used for analytical applications are oxidoreductase mimics, and their enzyme-like activities are usually demonstrated by using chromogenic and/or fluorogenic substrates. Herein, the phosphatase mimetic activity of cerium oxide nanoparticles (nanoceria) was investigated by using CDP-star as the chemiluminescent (CL) substrate. Interestingly, we found that the phosphatase mimetic activity of nanoceria can be remarkably inhibited by the addition of Al3+. Based on this finding, a highly sensitive and selective CL method for Al3+ detection is proposed. The CL intensity of the nanoceria/CDP-star system decreased with the increasing Al3+ concentrations in the range from 30 nM to 3.5 μM. A detection limit as low as 10 nM was obtained. Finally, the CL detection of intracellular Al3+ was achieved, demonstrating the utility of the CL method in complex biological samples.

中文翻译：

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基于氧化铈纳米颗粒的磷酸酶模拟活性，对细胞内Al3 +的高灵敏化学发光传感。

具有酶样特性的纳米材料（也称为纳米酶）在分析化学领域引起了越来越多的关注。然而，用于分析应用的大多数纳米酶是氧化还原酶模拟物，其酶样活性通常通过使用发色和/或发荧光的底物来证明。在本文中，通过使用CDP-star作为化学发光（CL）底物，研究了氧化铈纳米颗粒（nanoceria）的磷酸酶模拟活性。有趣的是，我们发现添加Al3 +可以明显抑制纳米氧化铈的磷酸酶模拟活性。基于这一发现，提出了一种用于Al3 +检测的高灵敏度，选择性CL方法。纳米氧化铈/ CDP-star系统的CL强度随着Al3 +浓度的增加而降低，范围为30 nM至3.5μM。获得的检测限低至10 nM。最终，实现了对细胞内Al3 +的CL检测，证明了CL方法在复杂生物样品中的实用性。