Effects of Intermittent versus Chronic-Moderate Ethanol Administration during Adolescence in the Adult Hippocampal Phosphoproteome.,Chemical Research in Toxicology

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Effects of Intermittent versus Chronic-Moderate Ethanol Administration during Adolescence in the Adult Hippocampal Phosphoproteome.
Chemical Research in Toxicology ( IF 3.7 ) Pub Date : 2020-01-23 , DOI: 10.1021/acs.chemrestox.9b00359
Ana Contreras ₁ , Lidia Morales ₁ , Nuria Del Olmo ₁ , Carmen Pérez-García ₁

Affiliation

Alcohol consumption during adolescence is known to cause different impairments in the hippocampus that could lead to persistent deficits in adulthood. A common pattern of alcohol use in adolescents consists of excessive and intermittent alcohol consumption over a very short period of time (binge drinking). Protein phosphorylation is a mechanism underlying memory processes and we have previously demonstrated changes in the rat hippocampal phosphoproteome after a single dose of ethanol; however, studies showing the phosphoprotein alterations in the hippocampus after repeated exposition to alcohol are limited. This study focuses on the identification of the phosphoproteins differentially regulated in the adolescent rat hippocampus after repeated ethanol administration by comparing different patterns of alcohol treatments according to dose and frequency of administration ((i) moderate dose-chronic use, (ii) low dose-intermittent use, and (iii) high dose-intermittent use). We have used a proteomic approach, including phosphoprotein enrichment by immobilized metal affinity chromatography, which revealed 21 proteins differentially affected depending on the pattern of alcohol treatment used. Many of these proteins are included in glycolysis and glucagon signaling pathways and are also involved in neurodegeneration, which could reinforce the role of metabolic alterations in the neural damage induced by repeated alcohol exposure during adolescence.

中文翻译：

成年海马磷酸化蛋白质组在青春期间歇性与慢性-中度乙醇管理的影响。

已知青春期饮酒会导致海马不同程度的损伤，可能导致成年期持续性赤字。青少年饮酒的常见模式包括在很短的时间内过量和间歇性饮酒（饮酒）。蛋白质磷酸化是记忆过程的基础机制，我们先前已经证明了单剂量乙醇后大鼠海马磷酸化蛋白质组的变化。然而，研究表明，反复暴露于酒精后，海马中的磷蛋白发生了改变。这项研究的重点是通过根据剂量和给药频率比较酒精治疗的不同方式，来确定重复给药后青春期大鼠海马中磷酸化蛋白的差异调节（（i）适度长期使用；（ii）低剂量-间歇使用，以及（iii）高剂量间歇使用）。我们已经使用了蛋白质组学方法，包括通过固定金属亲和色谱法富集磷蛋白，该方法揭示了21种蛋白质的差异取决于所用酒精处理的模式。这些蛋白质中的许多蛋白质都包含在糖酵解和胰高血糖素的信号传导途径中，并且还参与神经退行性变，这可能会增强代谢改变在青春期反复饮酒引起的神经损伤中的作用。

更新日期：2020-01-24

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