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A Thixotropic, Cell-Infiltrative Nanocellulose Hydrogel That Promotes in Vivo Tissue Remodeling
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2020-01-27 , DOI: 10.1021/acsbiomaterials.9b01549
Akihiro Nishiguchi 1 , Tetsushi Taguchi 1
Affiliation  

Injectable gels have been used in minimally invasive surgery for tissue regeneration and treatment of inflammatory diseases. However, polymeric hydrogels often fail in cell infiltration, because of the presence of dense, cross-linked molecular networks and a lack of bioactivity, which causes delayed tissue remodeling. Here, we report a thixotropic, cell-infiltrative hydrogel of biofunctionalized nanocellulose that topologically enhances cell infiltration and biochemically upregulates cellular activity for the promotion of tissue remodeling. Biodegradable, sulfonated nanocellulose forms a nanofibrous hydrogel, mimicking cellular microenvironments through cross-linking between nanocellulose and gelatin. Resulting nanocellulose hydrogels showed thixotropy, allowing for single syringe injection. Nanofiber-based hydrogels possess high molecular permeability, which is due to nanoporous structures. Sulfonate groups on nanocellulose increase protein adsorption and induce cellular extension in vitro. Highly sulfonated nanocellulose hydrogels enhanced cell infiltration and vascularization upon implantation into rats. Macrophage polarization to M2 was observed in nanocellulose hydrogels, which may be involved in tissue remodeling. Injectable, biofunctionalized nanocellulose gels have enormous potential as artificial biomatrices to heal inflammatory diseases through manipulation of the immune system and promotion of tissue remodeling.

中文翻译:

促进体内组织重塑的触变细胞浸润性纳米纤维素水凝胶

可注射凝胶已用于微创手术中,用于组织再生和炎性疾病的治疗。然而,由于存在致密的,交联的分子网络和缺乏生物活性的聚合物水凝胶通常无法使细胞浸润,从而导致组织重塑延迟。在这里,我们报告生物功能化的纳米纤维素的触变,细胞浸润水凝胶,拓扑上增强细胞浸润和生化上调细胞活性,以促进组织重塑。可生物降解的磺化纳米纤维素形成纳米纤维水凝胶,通过纳米纤维素和明胶之间的交联模拟细胞微环境。所得的纳米纤维素水凝胶显示触变性,允许单针筒注射。基于纳米纤维的水凝胶具有高分子渗透性,这归因于纳米孔结构。纳米纤维素上的磺酸根基团增加蛋白质的吸附并诱导体外细胞扩展。高度磺化的纳米纤维素水凝胶在植入大鼠体内后可增强细胞浸润和血管形成。在纳米纤维素水凝胶中观察到巨噬细胞向M2极化,这可能与组织重塑有关。可注射的生物功能化纳米纤维素凝胶具有巨大的潜力,可作为人造生物基质通过控制免疫系统和促进组织重塑来治愈炎症性疾病。高度磺化的纳米纤维素水凝胶在植入大鼠体内后可增强细胞浸润和血管形成。在纳米纤维素水凝胶中观察到巨噬细胞向M2极化,这可能与组织重塑有关。可注射的生物功能化纳米纤维素凝胶具有巨大的潜力,可作为人造生物基质通过控制免疫系统和促进组织重塑来治愈炎症性疾病。高度磺化的纳米纤维素水凝胶在植入大鼠体内后可增强细胞浸润和血管形成。在纳米纤维素水凝胶中观察到巨噬细胞向M2极化,这可能与组织重塑有关。可注射的生物功能化纳米纤维素凝胶具有巨大的潜力,可作为人造生物基质通过控制免疫系统和促进组织重塑来治愈炎症性疾病。
更新日期:2020-01-27
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