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Virulent coliphages in 1-year-old children fecal samples are fewer, but more infectious than temperate coliphages.
Nature Communications ( IF 14.7 ) Pub Date : 2020-01-17 , DOI: 10.1038/s41467-019-14042-z Aurélie Mathieu 1 , Moïra Dion 2, 3 , Ling Deng 4 , Denise Tremblay 3, 5 , Elisabeth Moncaut 1 , Shiraz A Shah 6 , Jakob Stokholm 6 , Karen A Krogfelt 7 , Susanne Schjørring 7 , Hans Bisgaard 6 , Dennis S Nielsen 4 , Sylvain Moineau 2, 3, 5 , Marie-Agnès Petit 1
Nature Communications ( IF 14.7 ) Pub Date : 2020-01-17 , DOI: 10.1038/s41467-019-14042-z Aurélie Mathieu 1 , Moïra Dion 2, 3 , Ling Deng 4 , Denise Tremblay 3, 5 , Elisabeth Moncaut 1 , Shiraz A Shah 6 , Jakob Stokholm 6 , Karen A Krogfelt 7 , Susanne Schjørring 7 , Hans Bisgaard 6 , Dennis S Nielsen 4 , Sylvain Moineau 2, 3, 5 , Marie-Agnès Petit 1
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Bacteriophages constitute an important part of the human gut microbiota, but their impact on this community is largely unknown. Here, we cultivate temperate phages produced by 900 E. coli strains isolated from 648 fecal samples from 1-year-old children and obtain coliphages directly from the viral fraction of the same fecal samples. We find that 63% of strains hosted phages, while 24% of the viromes contain phages targeting E. coli. 150 of these phages, half recovered from strain supernatants, half from virome (73% temperate and 27% virulent) were tested for their host range on 75 E. coli strains isolated from the same cohort. Temperate phages barely infected the gut strains, whereas virulent phages killed up to 68% of them. We conclude that in fecal samples from children, temperate coliphages dominate, while virulent ones have greater infectivity and broader host range, likely playing a role in gut microbiota dynamics.
中文翻译：
1岁儿童粪便样品中的强力噬菌体较少，但比温带的噬菌体更具感染性。
噬菌体是人类肠道菌群的重要组成部分，但它们对该社区的影响在很大程度上尚不清楚。在这里，我们培养了900株大肠杆菌的温和噬菌体，这些菌株是从1岁儿童的648份粪便样本中分离出来的，并直接从相同粪便样本的病毒级分中获得了噬菌体。我们发现63％的菌株带有噬菌体，而24％的病毒含有针对大肠杆菌的噬菌体。在从同一队列分离出的75株大肠杆菌中测试了其中150种噬菌体的宿主范围，这些噬菌体从菌株上清液中回收一半，从病毒体（73％温带，27％毒性）中分离。温和的噬菌体几乎没有感染肠道菌株，而有毒的噬菌体杀死了多达68％。我们得出的结论是，在儿童粪便样本中，温带性噬菌体占主导地位，
更新日期：2020-01-17
中文翻译：
1岁儿童粪便样品中的强力噬菌体较少，但比温带的噬菌体更具感染性。
噬菌体是人类肠道菌群的重要组成部分，但它们对该社区的影响在很大程度上尚不清楚。在这里，我们培养了900株大肠杆菌的温和噬菌体，这些菌株是从1岁儿童的648份粪便样本中分离出来的，并直接从相同粪便样本的病毒级分中获得了噬菌体。我们发现63％的菌株带有噬菌体，而24％的病毒含有针对大肠杆菌的噬菌体。在从同一队列分离出的75株大肠杆菌中测试了其中150种噬菌体的宿主范围，这些噬菌体从菌株上清液中回收一半，从病毒体（73％温带，27％毒性）中分离。温和的噬菌体几乎没有感染肠道菌株，而有毒的噬菌体杀死了多达68％。我们得出的结论是，在儿童粪便样本中，温带性噬菌体占主导地位，
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