当前位置: X-MOL 学术Sci. Rep. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Differential interaction of Or proteins with the PSY enzymes in saffron.
Scientific Reports ( IF 3.8 ) Pub Date : 2020-01-17 , DOI: 10.1038/s41598-020-57480-2
Oussama Ahrazem 1 , Alberto José López 1 , Javier Argandoña 1 , Raquel Castillo 2 , Ángela Rubio-Moraga 1 , Lourdes Gómez-Gómez 1
Affiliation  

Colored apocarotenoids accumulate at high concentrations in few plant species, where display a role in attraction of pollinators and seed dispersers. Among these apocarotenoids, crocins accumulate at high concentrations in the stigma of saffron and are responsible for the organoleptic and medicinal properties of this spice. Phytoene synthase and Orange protein are key for carotenoid biosynthesis and accumulation. We previously isolated four phytoene synthase genes from saffron with differential roles in carotenoid and apocarotenoid biosynthesis. However, the implications of Orange genes in the regulation of apocarotenoid accumulation are unknown. Here, we have identified two Orange genes from saffron, with different expression patterns. CsOr-a was mainly expressed in vegetative tissues and was induced by light and repressed by heat stress. Both CsOr-a and CsOr-b were expressed in stigmas but showed a different profile during the development of this tissue. The interactions of CsOr-a and CsOr-b were tested with all the four phytoene synthase proteins from saffron and with CsCCD2. None interactions were detected with CCD2 neither with the phytoene synthase 2, involved in apocarotenoid biosynthesis in saffron. The obtained results provide evidence of different mechanisms regulating the phytoene synthase enzymes in saffron by Orange for carotenoid and apocarotenoid accumulation in saffron.

中文翻译:

藏红花中Or蛋白质与PSY酶的差异相互作用。

有色的类胡萝卜素在少数植物中高浓度积累,在这些植物中显示出对传粉媒介和种子分散剂的吸引作用。在这些类胡萝卜素中,藏红花在藏红花的柱头中以高浓度积聚,是造成这种香料的感官和医学特性的原因。番茄红素合酶和橙蛋白是类胡萝卜素生物合成和积累的关键。我们以前从藏红花中分离了四个八氢番茄红素合酶基因,它们在类胡萝卜素和类胡萝卜素的生物合成中具有不同的作用。但是,Orange基因在类胡萝卜素积累调控中的意义尚不清楚。在这里,我们从藏红花中鉴定了两个橙色基因,它们具有不同的表达模式。CsOr-a主要在营养组织中表达,受光诱导,受热胁迫抑制。CsOr-a和CsOr-b均以柱头表达,但在该组织发育过程中显示出不同的轮廓。CsOr-a和CsOr-b的相互作用与藏红花中的所有四种phytoene合酶蛋白以及CsCCD2进行了测试。没有检测到与参与藏红花中类胡萝卜素生物合成的八氢番茄红素合酶2的CCD2相互作用。所得结果提供了橙色调节藏红花中的植物生长素合酶对藏红花中类胡萝卜素和类胡萝卜素积累的不同机制的证据。没有检测到与参与藏红花中类胡萝卜素生物合成的八氢番茄红素合酶2的CCD2相互作用。所得结果提供了橙色调节藏红花中的植物生长素合酶对藏红花中类胡萝卜素和类胡萝卜素积累的不同机制的证据。没有检测到与参与藏红花中类胡萝卜素生物合成的八氢番茄红素合酶2的CCD2相互作用。所得结果提供了橙色调节藏红花中的植物生长素合酶对藏红花中类胡萝卜素和类胡萝卜素积累的不同机制的证据。
更新日期:2020-01-17
down
wechat
bug