Abstract Despite the evident importance of arbuscular mycorrhizal fungi (AM fungal) associated with the root and rhizosphere system of elevation gradients, limit is known about the difference in AM fungal diversity between root and rhizosphere and their influencing factors along elevation gradients. AM fungal Here, we designed an elevation gradient experiment, which covered six elevations and three vegetation types, and collected plant and soil samples. We used Illumina gene sequencing to analyze AM fungal diversity in roots and rhizosphere soils; other potential factors, such as plant diversity, leaf C, N, and P, litter, soil and microbial biomass, extracellular enzymes activity, and the bacterial and ITS broad-fungal diversity, were also determined. We found that AM fungal diversity differences between root and rhizosphere soil diminished as elevation increased, despite that AM fungal diversity had a well-known pattern with elevation (unimodal patterns). Compared with plant characteristics, soil properties (soil C, N, P, C:N, C:P, N:P) were stronger effect on AM fungal diversity between root and rhizosphere. Particularly, soil extracellular enzymes activity and bacterial and ITS broad-fungal diversity explained more variation in AM fungal diversity dynamics along the elevation gradients. Our findings indicate that the diminishment of AM fungal diversity in root and rhizosphere soil are a response to elevation gradients through changes of soil extracellular enzymes activity and the bacterial and fungi communities, which provided evidence that AM fungal community dynamics link to climate change.

中文翻译：

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海拔梯度影响根际土壤丛枝菌根真菌多样性差异

摘要 尽管丛枝菌根真菌 (AM fungal) 与海拔梯度的根和根际系统相关联的重要性明显，但根和根际之间 AM 真菌多样性的差异及其影响因素沿海拔梯度的影响尚不清楚。AM 真菌 在这里，我们设计了一个海拔梯度实验，覆盖了六个海拔和三种植被类型，并收集了植物和土壤样本。我们使用 Illumina 基因测序来分析根和根际土壤中 AM 真菌的多样性；还确定了其他潜在因素，如植物多样性、叶 C、N 和 P、凋落物、土壤和微生物生物量、细胞外酶活性以及细菌和 ITS 广泛真菌多样性。我们发现根和根际土壤之间的 AM 真菌多样性差异随着海拔的增加而减少，尽管 AM 真菌多样性具有众所周知的海拔模式（单峰模式）。与植物特性相比，土壤性质（土壤 C、N、P、C:N、C:P、N:P）对根和根际 AM 真菌多样性的影响更大。特别是，土壤细胞外酶活性以及细菌和 ITS 广泛真菌多样性解释了 AM 真菌多样性动态沿海拔梯度的更多变化。我们的研究结果表明，根和根际土壤中 AM 真菌多样性的减少是通过土壤细胞外酶活性和细菌和真菌群落变化对海拔梯度的响应，