Selenoprotein F (Selenof) is an endoplasmic reticulum (ER)-resident protein. It may be functionally linked to glycoprotein folding in the ER but the detail function is not fully understood. To study the function of Selenof, we used CRISPR/Cas9 to generate Selenof knockout mice and performed proteomic analysis of hepatic proteins by iTRAQ. Collectively, 83 differently expressed proteins (DEPs) were identified in the liver of Selenof knockout mice. The changes of mRNA and protein levels of 6 selected DEPs, Fatty acid synthase, ATP-citrate synthase, Glutathione S-transferase P 1, Transformer-2 protein homolog beta, Pyruvate kinase, Metallothionein-2, were further verified by quantitative real-time PCR or Western blot. The roles of 83 DEPs are mainly related to metabolism and cancer. Consistently, the levels of NADPH and ATP, two molecules closely related with energy metabolism, are significantly changed in the livers of Selenof knockout mice.

Significance

Our study identified potential biological pathways and proteins related to Selenof deletion. These findings will provide possible proteins/pathways related to Selenof and help to understand the function of Selenof and its relationship with certain diseases.

中文翻译：

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iTRAQ 对硒蛋白 F 敲除小鼠的肝脏蛋白质组学分析：对硒蛋白 F 在代谢和疾病中的作用的启示。

硒蛋白 F (Selenof) 是一种内质网 (ER) 驻留蛋白。它可能在功能上与 ER 中的糖蛋白折叠有关，但详细功能尚不完全清楚。为了研究 Selenof 的功能，我们使用 CRISPR/Cas9 生成了 Selenof 敲除小鼠，并通过 iTRAQ 对肝脏蛋白进行了蛋白质组学分析。总的来说，在 Selenof 敲除小鼠的肝脏中鉴定出 83 种不同表达的蛋白质 (DEP)。通过实时定量进一步验证了 6 个选定的 DEP、脂肪酸合酶、ATP-柠檬酸合酶、谷胱甘肽 S-转移酶 P 1、Transformer-2 蛋白同源物 β、丙酮酸激酶、金属硫蛋白 2 的 mRNA 和蛋白质水平的变化PCR 或蛋白质印迹。83个DEP的作用主要与代谢和癌症有关。一致地，NADPH 和 ATP 的水平，

意义

我们的研究确定了与 Selenof 缺失相关的潜在生物学途径和蛋白质。这些发现将提供可能与 Selenof 相关的蛋白质/途径，并有助于了解 Selenof 的功能及其与某些疾病的关系。